Quantification of endothelin-1 level in placentae and human fetal membranes

Authors

  • Agnes Vânia Apolinário Universidade de Brasília, Brasília, DF
  • Maria Angélica Miglino Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Cirurgia, São Paulo, SP
  • Yolanda Galindo Pacheco Universidade de Brasília, Brasília, DF
  • Flávia T. Verechia Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Cirurgia, São Paulo, SP
  • Carlos Eduardo Ambrosio Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Cirurgia, São Paulo, SP

DOI:

https://doi.org/10.1590/S1413-95962003000900005

Keywords:

Human placenta, Endothelin-1, Fetal membranes, Twin birth

Abstract

The endothelin is a potent vasoconstricor that possess angiogenic, mitogenic and neurotrophical functions. In this study we aimed to quantify the Endothelin-1 (ET-1) levels in chorionic placental villi and amnion of placentae of twins childbirths whose women were submitted to an assisted pregnancy and spontaneal fecundation. Were collected 08 placental samples. After the placental delivery the samples had been placed in liquid nitrogen and later in a -80ºC until the Enzyme-Linked Immunosorbent Assay (ELISA) technique. The concentration levels of ET-1 in samples of chorionic villi in twins placentae varied from 0,52 to 0,70 fmol/ml, while in chorionic villi single gestation varied from 0,47 to 0,86 fmol/ml. The same determination in samples of amnion of twins placentae varied from 0,65 to 1,16 fmol/ml, while in single gestation varied from 0,65 to 1,04 fmol/ml. These findings suggests that in the chorionic villi the levels of ET-1 didn't vary between twins and single gestation. In the amnion the levels of ET-1 are higher in twins than in single gestation, suggesting that the amnion is the source of ET-1 in the amniotic fluid.

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Published

2003-01-01

How to Cite

Apolinário, A. V., Miglino, M. A., Pacheco, Y. G., Verechia, F. T., & Ambrosio, C. E. (2003). Quantification of endothelin-1 level in placentae and human fetal membranes. Brazilian Journal of Veterinary Research and Animal Science, 40(supl.), 185-190. https://doi.org/10.1590/S1413-95962003000900005

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