Endometrial prostaglandin F2α in vitro production and its modulation regarding dominant follicle position in cattle

  • Fabiana Fernandes Bressan Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal; Universidade de São Paulo, Faculdade de Zootecnia e Engenharia de Alimentos da USP, Departamento de Medicina Veterinária
  • Claudia Maria Bertan Membrive Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal; Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Ciências Agrárias e Tecnológicas
  • Marcelo Demarchi Goissis Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal
  • Vanessa Belentani Marques Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal
  • Pauline Martins da Cunha Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal
  • Mario Binelli Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal
Keywords: Cattle, Luteolysis, PGF2α synthesis, Reproductive physiology

Abstract

Prostaglandin F2α (PGF2α) determines luteolysis in cattle, and the ability to manipulate its endogenous synthesis is indispensible for large-scale animal breeding. Estradiol (E2) and progesterone (P4) modulate several molecular pathways in endometrial cells, including the synthesis of PGF2α; however, its specific mechanisms are still not totally known. This study investigated the production in vitro and possible modulation of endometrial PGF2α due to a local effect of endogenous E2 in the ipsilateral uterine horn (UH) containing the dominant follicle (DF) or from P4 in ipsilateral horn containing the corpus luteum (CL). The PGF2α stimulators oxytocin (OT) and phorbol 12,13-dibutyrate (PDBu) were incubated with endometrial explants, and PGF2α content was measured. For that, cycling cows were synchronized, the development of DF and CL was examined by ultrasonography and on the seventh day of the estrous cycle, endometrial explants were collected and cultured in medium supplemented with 10-6 M PDBu or 10-6 M OT or non-supplemented. Media samples were collected immediately after treatment and 60 min later. Radioimmunoassay showed that the PGF2α content of the UH ipsilateral to the DF was 49% less than that of the contralateral UH (8.22 ± 0.95 vs. 12.24 ± 0.95 pg/mL/mg tissue, respectively; P < 0.01). However, the PGF2α levels did not differ between the UHs as a function of the CL position (9.46 ± 0.95 vs. 11 ± 0.95 pg/mL/mg; P > 0.05). The cellular stimulators promoted an increase in PGF2α synthesis (P < 0.02), and the effects differed among the animals (P < 0.04). The PGF2a production was higher in the explants treated with PDBu rather than OT (13.68 ± 1.16 vs. 10.01 ± 1.16 pg/mL/mg tissue, respectively; P < 0.05). In conclusion, PGF2α synthesis is modulated by the presence of the DF (local E2) but not the CL (local P4), and both PDBu and OT stimulated PGF2a synthesis.

Downloads

Download data is not yet available.
Published
2018-07-26
How to Cite
Bressan, F., Membrive, C. M., Goissis, M., Marques, V., Cunha, P., & Binelli, M. (2018). Endometrial prostaglandin F2α in vitro production and its modulation regarding dominant follicle position in cattle. Brazilian Journal of Veterinary Research and Animal Science, 55(2), e133937. https://doi.org/10.11606/issn.1678-4456.bjvras.2018.133937
Section
FULL ARTICLE