Can centrifugation force compromise the plasmatic membrane, acrosome and DNA integrity of goat spermatozoa?

Authors

  • André Maciel Crespilho Universidade Santo Amaro; Universidade Severino Sombra; Vetsemen® - Análise de sêmen para Inseminação Artificial
  • Karinne Ávila Bosco Universidade Santo Amaro
  • Camila de Paula Freitas Dell'Aqua Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Medicina Veterinária e Zootecnia
  • Lorenzo Garrido Segabinazzi Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Medicina Veterinária e Zootecnia
  • Frederico Ozanam Papa Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Medicina Veterinária e Zootecnia
  • Karoline Maria Gil Brás Universidade Santo Amaro
  • Gustavo Mendes Gomes Universidade Severino Sombra
  • Kleber da Cunha Peixoto Junior Universidade Santo Amaro

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2018.145873

Keywords:

Centrifugation, Goat, Integrity, Semen, Viability

Abstract

Protocols for cooling or freezing goat semen usually recommend centrifugation for seminal plasma removal. However, little is known about the effect of this process on goat sperm viability and functionality. The present study evaluated the effects of centrifugation force on the plasma membrane, acrosomes, and DNA integrity of goat semen. Four ejaculates from each of the four different Anglo Nubian male goats were used. Semen samples were obtained using artificial vagina, and immediately after collection, ejaculates were diluted using Ringer’s sodium lactate solution and split into three groups: Control (CG, without centrifugation), G1 (centrifugation 600 x g/10 min), G2 (centrifugation 1200 x g/10 min). After centrifugation, seminal plasma was removed, the sperm pellets were resuspended using Tris-egg yolk extender (80 x 106 spermatozoa/mL) and the sperm morphology was analyzed. Samples were cooled at 5°C for 5, 24, 36, and 48 h and then sperm plasma membrane and acrosome integrity (PMAI, %) and sperm DNA fragmentation index (SDF, %) were evaluated at each time-point, using a flow cytometer. Additionally, sperm movement was determined using computer semen analysis (CASA) after 5, 24, and 48 h of refrigeration period. The semen centrifugation did not induce additional sperm morphology defect or reduction in sperm kinetics in the experimental groups. Differences were not observed (p > 0.05) in PMAI and SDF among different groups, in any of each timepoint
of the cooling process. In conclusion, centrifugation, even at high speeds, did not affect goat sperm integrity and functionality when submitted to refrigeration process.

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Published

2018-12-12

Issue

Section

FULL ARTICLE

How to Cite

1.
Crespilho AM, Bosco K Ávila, Dell'Aqua C de PF, Segabinazzi LG, Papa FO, Brás KMG, et al. Can centrifugation force compromise the plasmatic membrane, acrosome and DNA integrity of goat spermatozoa?. Braz. J. Vet. Res. Anim. Sci. [Internet]. 2018 Dec. 12 [cited 2024 May 26];55(3):e145873. Available from: https://www.revistas.usp.br/bjvras/article/view/145873