Blood typing in positive DEA 1 dogs

comparative analysis between immunochromatography, hemagglutination and flow cytometry

Authors

  • Suzana Claudia Spinola Santos Universidade Federal da Bahia. Instituto de Ciências da Saúde http://orcid.org/0000-0001-5852-3689
  • Mariane Melo Santos Universidade Federal da Bahia. Instituto de Ciências da Saúde. Laboratório de Imunologia e Biologia Molecular, Salvador – BA, Brazil
  • Wellington Francisco Rodrigues Universidade Federal do Triângulo Mineiro
  • Roberto Meyer Universidade Federal da Bahia. Instituto de Ciências da Saúde
  • Maria de Fátima Dias Costa Universidade Federal da Bahia. Instituto de Ciências da Saúde

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2020.151444

Keywords:

Canine transfusion medicine, Transfusion risk in dogs, Dog erythrocyte antigen 1, Flow cytometry

Abstract

Blood typing techniques have been improved to ensure greater safety for transfusion procedures. Typification for the DEA 1 antigen through flow cytometry should offer more reliability to routine immunohematology in donor and recipient dogs. Currently, the DEA 1 group is starting to be an autosomal dominant allelic system with the DEA 1 negative type and its variations of positivity. The present study investigated the DEA 1 antigen using the techniques of immunochromatography, hemagglutination and flow cytometry. Among the positive animals for the DEA 1 group, typified by flow cytometry, medium intensities of fluorescence were found, which are indicative of weak, moderate and strong antigenicity. This enabled the division of the DEA 1 group into weak positive, moderate positive and strong positive. The blood typing techniques for the DEA 1 group by flow cytometry, agglutination and immunochromatography had positive (Spearman r=0.70) and statistically significant (p>0.0001) correlations.

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Published

2020-03-30

How to Cite

Santos, S. C. S., Santos, M. M., Rodrigues, W. F., Meyer, R., & Costa, M. de F. D. (2020). Blood typing in positive DEA 1 dogs: comparative analysis between immunochromatography, hemagglutination and flow cytometry. Brazilian Journal of Veterinary Research and Animal Science, 57(1), e151444. https://doi.org/10.11606/issn.1678-4456.bjvras.2020.151444

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