Buffaloes (Bubalus bubalis) mycotic eye infection: reported herd cases in Thi-Qar province, Iraq

Authors

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2021.180221

Keywords:

Bubaline, Fungal eye infections, Aspergillus flavus, Mucor spp.

Abstract

Buffaloes are one of the important farm animals in the south of Iraq and play an essential economical role mainly acting as dairy, meat, and draft animals. This study intended to diagnose buffalo mycotic eye infections in Thi-Qar province/Iraq. Some 250 buffaloes in the herd of 3,700 animals suffered from eye infections from December 2017 to November 2018. Eye swabs were collected from each infected eye of the affected buffaloes of both sexes before treatment. The animals were in different age groups. All samples were transferred to the laboratory in transfer media, and cultured on Sabouraud dextrose (SDA) agar with and without 0.05 g/mL and 0.4 g/mL chloramphenicol and cycloheximide, respectively. Later, the agars were incubated at 25o C and 37o C. The total percentage of eye infection was (6.75%), constituting (49.2%) mycotic infections. The predominant clinical manifestations that appeared on the infected buffaloes were eye inflammation represented by congestion, lacrimation, the opacity of cornea and edema, and reduced productivity of the infected animals. Different fungal isolates were identified from the samples including Aspergillus fumigates, Aspergillus flavus, Aspergillus niger, Penicillium spp., Alternaria spp., Fusarium spp., Candida spp., Cladosporium spp., Rhodotorula spp., Mucor spp. and Rhizopus spp. Calves buffaloes below one-year-old were more prone to mycotic infection than one-year-old or more. Additionally, male buffaloes were more susceptible to infection than females. In conclusion, this study isolated various types of fungus from the inflamed eyes of buffaloes. Fungal eye infection and the potential risk factors for fungal keratitis in buffaloes were also investigated. The study also approved the rapid diagnosis of fungi by direct microscopic detection and culture. The author recommends future studies including large numbers of the buffalo herd in Iraq to determine the epidemiology of this condition in the country. 

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References

Acharya Y, Acharya B, Karki P. Fungal keratitis: study of increasing trend and common determinants. Nepal J Epidemiol. 2017;7(2):685-93. http://dx.doi.org/10.3126/nje.v7i2.17975. PMid:29181230.

Andrew SE, Nguyen A, Jones GL, Brooks DE. Seasonal effect on the aerobic bacterial and fungal conjunctival flora of normal thorough breed brood mares in Florida. Vet Ophthalmol. 2003;6(1):45-50. http://dx.doi.org/10.1046/j.1463-5224.2003.00265.x. PMid:12641842.

Bandyopadhyay S, Das D, Mondal KK, Ghanta AK, Purkrit SK, Bhasrar R. Epidemiology and laboratory diagnosis of fungal corneal ulcer in the Sundarban Region of West Bengal, eastern India. Nepal J Ophthalmol. 2012;4(1):29- 36. http://dx.doi.org/10.3126/nepjoph.v4i1.5847.

Behdad A, Araghi-Sooreh A. Investigation of fungal isolated of normal conjunctival sac in the Turkmen (Yamud) Horses. J Vet Micro. 2014;10(1):65-43.

Bond R, Morris DO, Guillot J, Bensignor EJ, Robson D, Mason KV, Kano D, Hill PB. Biology, diagnosis and treatment of Malassezia dermatitis in dogs and cats Clinical Consensus Guidelines of the World Association for Veterinary Dermatology. Vet Dermatol. 2020;31(1):27-e4.

Borghese A, Moioli B. Buffalo: mediteranean region. Encyclopedia of Dairy Sciences. 2011;1:780-4. Bourguet A, Desprez I, Volait L, Guyonnet A, Pignon C, Boulouis HJ, Guillot J, Chahory S. Conjunctival bacterial and fungal flora and cutaneous fungal flora in healthy domestic rabbits (Oryctolagus cuniculus). J Small Anim Pract. 2019;60(7):417-22. http://dx.doi.org/10.1111/jsap.12989. PMid:30875093.

Dóczi I, Gyetvai T, Kredics L, Nagy E. Involvement of Fusarium spp. In fungal keratitis. Clin Microbiol Infect. 2004;10(9):773-6. http://dx.doi.org/10.1111/j.1469-0691.2004.00909.x. PMid:15355406.

Ge C, Wei C, Yang BX, Cheng J, Huang YS. Conjunctival microbiome changes associated with fungal keratitis: metagenomic analysis. Int J Ophthalmol. 2019;12(2):194- 200. PMid:30809472. Gilger BC. Immunology of ocular surface. Vet Clin North Am Small Anim Pract. 2008;38(2):223-31, v. http://dx.doi.org/10.1016/j.cvsm.2007.11.004. PMid:18299004.

Handool HH. Isolation and identification of some genera and species of bacteria and fungi from conjunctiva in cattle in AL-Diwaniya. AL –Qadisiya journal of Vet. Med Sci. 2013;12(2):55-62.

Johns IC, Baxter K, Booler H, Hicks C, Menzies-Gow N. Conjunctival bacterial and fungal flora in healthy horses in the UK. Vet Ophthalmol. 2011;14(3):195-9. http://dx.doi.org/10.1111/j.1463-5224.2010.00867.x. PMid:21521444.

Kalyankar SD, Chavan AA, Khedkar CD, Kalyankar SP. Studies On Management Practices of Buffaloes In Different Agro-Climatic Zones Of Maharashtra. Indian J Anim Res. 2008;42(3):157-63.

Khosravi AR, Nikaein D, Sharifzadeh A, Gharagozlou F. Ocular fungi flora from healthy horses in Iran. J Mycol Med. 2014;24(1):29-33. http://dx.doi.org/10.1016/j.mycmed.2013.10.006. PMid:24411178.

Klotz SA, Penn CC, Negvesky GJ, Butrus SI. Fungal and parasitic infections of the eye. Clin Microbiol Rev. 2000;13(4):662-85. http://dx.doi.org/10.1128/CMR.13.4.662. PMid:11023963.

Kumar A, Khurana A, Sharma M, Chauhan L. Causative fungi and treatment outcome of dematiaceous fungal keratitis in North India. Indian J Ophthalmol. 2019;67(7):1048-53. http://dx.doi.org/10.4103/ijo.IJO_1612_18. PMid:31238406.

Lamagna B, Pasolini MP, Nizza S, Mallardo K, Formicola M, Costagliola A, Fatone G, Fiorito F, Paciello O, De Martino L. Conjunctival cytological examination, bacteriological culture, and antimicrobial resistance profiles of healthy Mediterranean buffaloes (Bubalus bubalis) from Southern Italy. Asian Pac J Trop Biomed. 2015;5(11):889-95. http://dx.doi.org/10.1016/j.apjtb.2015.06.017.

Ledbetter EC. Antifungal therapy in equine ocular mycotic infections. Vet Clin North Am Equine Pract. 2017;33(3):583-605. http://dx.doi.org/10.1016/j.cveq.2017.08.001. PMid:28958862.

Maxwell KM, Plummer CE, Brooks DE. The fungal plaque form of equine keratomycosis. Vet Med Anim Sci. 2015;3:1- 10. http://dx.doi.org/10.7243/2054-3425-3-2.

Nardoni S, Sgorbini M, Barsotti G, Corazza M, Mancianti F. (Counjunctival fungal flora in healthy donkey. Vet Ophthalmol. 2007;10(4):207-10. http://dx.doi.org/10.1111/j.1463-5224.2007.00537.x. PMid:17565551.

Pal M. First report of isolation Fusarium solani from equine keratitis in Gujarat, India. EC Microbiology. 2017;9(5):174-6.

Pinard CL, Brightman AH, Yeary TJ, Everson TD, Cox LK, Chengappa MM, Davidson HJ. Normal conjunctival flora in north America opossum (didelphis virginiana) and raccoon (procyon lotor). J Wildl Dis. 2002;38(4):851-5. http://dx.doi.org/10.7589/0090-3558-38.4.851. PMid:12528457.

Rajesh K, Suresh K, Syaamasunder N. Infectious bovine keratoconjunctivitis in abuffalo and therapeutic aspects. Buffalo Bull. 2009;28(3):110-2.

Samuelson DA, Andresen TL, Gwin RM. Conjunctival fungal flora in horses, cattle, dogs, and cats. Vet Ophthalmol. 2010;13(4):227-34.

Seyedmousavi S, Bosco SMG, de Hoog S, Ebel F, Elad D, Gomes RR, Jacobsen ID, Jensen HE, Martel A, Mignon B, Pasmans F, Piecková E, Rodrigues AM, Singh K, Vicente VA, Wibbelt G, Wiederhold NP, Guillot J. Fungal infections in animals: a patchwork of different situations. Med Mycol. 2018;1(56, suppl_1):165-87. http://dx.doi.org/10.1093/mmy/myx104. PMid:29538732.

Sgorbini M, Barsotti G, Nardoni S, Brombin M, Sbrana A, Mancianti F, Corazza M. Seasonal prevalence of fungi in the conjunctival fornix of healthy cows during a 2-year study. Vet Ophthalmol. 2010;13(4):227-34. http://dx.doi.org/10.1111/j.1463-5224.2010.00788.x.

Shivaji S, Jayasudha R, Sai Prashanthi G, Kalyana Chakravarthy S, Sharma S. The human ocular surface fungal microbiome. Invest Ophthalmol Vis Sci. 2019;60(1):451-9. PMid:30703210.

Sundaram BM, Badrinath S, Subramanian S. Studies on mycotic keratitis. Mycosis. 1989;32(11):568-72. http://dx.doi.org/10.1111/j.1439-0507.1989.tb02183.x. PMid:2615780.

Voelter-Ratson K, Monod M, Braun U, Spiess BM. Ulcerative fungal keratitis in brown swiss cow. Vet Ophthalmol. 2013;16(6):464-6. http://dx.doi.org/10.1111/vop.12037. PMid:23437914.

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Published

2021-07-14

How to Cite

Khalaf, J. M. ., Karim, A. J., & Al Salihi, K. A. (2021). Buffaloes (Bubalus bubalis) mycotic eye infection: reported herd cases in Thi-Qar province, Iraq. Brazilian Journal of Veterinary Research and Animal Science, 58, e180221. https://doi.org/10.11606/issn.1678-4456.bjvras.2021.180221

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