Prostaglandin E2 induces ovulation in prepubertal mice


  • Jéssica de Souza Andrade Rede Bionorte, Programa de Pós-graduação em Biodiversidade e Biotecnologia
  • Juliana Pavan Zuliani Fundação Oswaldo Cruz de Rondônia, Laboratório de Imunologia Aplicada à Saúde
  • Jaswant Singh University of Saskatchewan, Veterinary Biomedical Sciences
  • Sulamita da Silva Setúbal Fundação Oswaldo Cruz de Rondônia, Laboratório de Imunologia Aplicada à Saúde
  • Renata Reis da Silva Empresa Brasileira de Pesquisa Agropecuária
  • Augusto Schneider Universidade Federal de Pelotas
  • Luiz Francisco Machado Pfeifer Empresa Brasileira de Pesquisa Agropecuária



Ovulation, Prostaglandin, Receptor, Puberty


The objective of this study was to determine the ability of prostaglandin E2 (PGE2) to induce ovulation and expression of PGE2 receptor (EP2 and EP4) and COX genes (COX-1 and COX-2) in the ovary and pituitary of prepubertal mice. The positive control consisted of the application of 5 μg of gonadotropin-releasing hormone (GnRH, n = 29); the negative control applied 0.5 mL of phosphate buffered saline (PBS, n=31); the treatment tested the application of 250 μg of PGE2 (n = 29), making a total of 89 prepubertal mice (BALB/c). Mice were euthanized 14 to 15 h after treatments to detect ovulation and tissue collection. A Chi-square test was used to compare the proportion of animals ovulating. Gene expressions and number of ovulation were analyzed by one-way ANOVA and Tukey’s test was used to compare means among groups. A greater proportion of mice (P < 0.001) ovulated after receiving GnRH (89.7%, 26/29) compared to PGE2 group (58.6%, 17/29). However, the proportion was higher compared to those treated with PBS (0%, 0/31). Ep2 gene expression in the pituitary was > two-fold higher (P < 0.05) in the PGE2 group compared to the PBS and GnRH groups. Further, PGE2 stimulated Cox1 (2.7 fold, P < 0.05) while GnRH stimulated Cox2 expression (6.5 fold, P < 0.05) in the pituitary when compared to the PBS group. In conclusion, our results support the hypothesis that PGE2 can induce ovulation in prepubertal mice with a concomitant increase in Ep2 and Cox1 gene expression in the pituitary gland.


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How to Cite

Andrade, J. de S., Zuliani, J. P. ., Singh, J. ., Setúbal, S. da S. ., Silva, R. R. da, Schneider, A. ., & Pfeifer, L. F. M. . (2021). Prostaglandin E2 induces ovulation in prepubertal mice. Brazilian Journal of Veterinary Research and Animal Science, 58, e182745.




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