Canine gastrointestinal pythiosis

a case report in Brazilian Pantanal from a diagnostic approach

Authors

  • Daniel Moura de Aguiar Universidade Federal do Mato Grosso, Faculdade de Medicina Veterinária, Hospital Veterinário, Departamento de Doenças Infeciosas ; 2 Universidade Federal do Mato Grosso, Faculdade de Medicina Veterinária, Hospital Veterinário, Laboratório de Virologia e Rickettsioses http://orcid.org/0000-0001-8631-522X
  • Manoela Sifuentes Universidade Federal do Mato Grosso, Faculdade de Medicina Veterinária, Hospital Veterinário, Departamento de Doenças Infeciosas
  • Aline Magalhães Universidade Federal do Mato Grosso, Faculdade de Medicina Veterinária, Hospital Veterinário, Laboratório de Patologia Veterinária
  • Nathalia Pereira Universidade Federal do Mato Grosso, Faculdade de Medicina Veterinária, Hospital Veterinário, Laboratório de Virologia e Rickettsioses https://orcid.org/0000-0002-4045-9073
  • Caroline Pescador Universidade Federal do Mato Grosso, Faculdade de Medicina Veterinária, Hospital Veterinário, Laboratório de Patologia Veterinária https://orcid.org/0000-0002-2754-1478
  • Stéfano Cândido Universidade Federal do Mato Grosso, Faculdade de Medicina Veterinária, Hospital Veterinário, Laboratório de Microbiologia e Biologia Molecular https://orcid.org/0000-0003-2735-2121
  • Valéria Dutra Universidade Federal do Mato Grosso, Faculdade de Medicina Veterinária, Hospital Veterinário, Laboratório de Microbiologia e Biologia Molecular https://orcid.org/0000-0002-6630-2293
  • Luciano Nakazato Universidade Federal do Mato Grosso, Faculdade de Medicina Veterinária, Hospital Veterinário, Laboratório de Microbiologia e Biologia Molecular https://orcid.org/0000-0002-1244-0690
  • Uiara Barreto Universidade Federal do Mato Grosso, Faculdade de Medicina Veterinária, Hospital Veterinário, Serviço de Diagnóstico por Imagem
  • Mayara Burema Universidade Federal do Mato Grosso, Faculdade de Medicina Veterinária, Hospital Veterinário, Serviço de Diagnóstico por Imagem
  • Pedro Néspoli Universidade Federal do Mato Grosso, Faculdade de Medicina Veterinária, Hospital Veterinário, Serviço de Diagnóstico por Imagem https://orcid.org/0000-0001-9286-8891
  • Juliana Rodrigues Universidade Federal do Mato Grosso, Faculdade de Medicina Veterinária, Hospital Veterinário, Laboratório de Patologia Clínica

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2022.186005

Keywords:

Diarrhea, Pythium insidiosum, Mammals, Granulomatous disease, Necropsy

Abstract

Pythiosis is caused by an aquatic fungus-like organism (Pythium insidiosum). It is considered an important public health issue as it can affect both animals and humans. This paper reports a case of gastrointestinal pythiosis in a dog. The patient was hospitalized for four days, during which the animal received supportive and symptomatic treatment. But the applied treatment was unsuccessful and the dog’s clinical condition worsened, culminating in death. Complementary imaging tests such as radiography and ultrasonography, as well as hematological tests, were performed during the hospitalization period. The definitive diagnosis was reached in the postmortem as macroscopic and microscopic characteristics suggested the presence of intestinal granuloma and accentuated multifocal suppurative necrotic enteritis. Additionally, the histological evaluation revealed morphological structures compatible with P. insidiosum. Also, the results of nested PCR performed
showed partial amplification (105 bp) of the ITS1 region of the ribosomal gene of P. insidiosum.

Downloads

Download data is not yet available.

References

Bosco SMG, Hussni CA, Santurio JM, Bagagli E. Pitiose. In: Megid J, Ribeiro MG, Paes AC, editors. Doenças infecciosas em animais de produção e de companhia, Rio de Janeiro: ROCA; 2016, pp. 946-57.

De Cock AW, Mendoza L, Padhye AA, Ajello L, Kaufman L. Pythium insidiosum sp. nov., the etiologic agent of pythiosis. J Clin Microbiol. 1987;25(2):344-9. http://dx.doi.org/10.1128/jcm.25.2.344-349.1987. PMid:3818928.

Feldman BV, Zinkl JG, Jain NC, Schalm OW. Schalm’s veterinary hematology. 6th ed. New Jersey: Wiley-Blackwell; 2016.

Fischer JR, Pace LW, Turk JR, Kreeger JM, Miller MA, Gosser HS. Gastrointestinal pythiosis in Missouri dogs: eleven cases. J Vet Diagn Invest. 1994;6(3):380-2. http://dx.doi.org/10.1177/104063879400600320. PMid:7948214.

Francino O, Altet L, Sánchez-Robert E, Rodriguez A, Solano-Gallego L, Alberola J, Ferrer L, Sánchez A, Roura X. Advantages of real-time PCR assay for diagnosis and monitoring of canine leishmaniosis. Vet Parasitol. 2006;137(3- 4):214-21. http://dx.doi.org/10.1016/j.vetpar.2006.01.011. PMid:16473467.

Gaastra W, Lipman LJA, De Cock AWAM, Exel TK, Pegge RBG, Scheurwater J, Vilela R, Mendoza L. Pythium insidiosum: an overview. Vet Microbiol. 2010;146(1-2):1-16. http://dx.doi.org/10.1016/j.vetmic.2010.07.019. PMid:20800978.

Grooters AM, Gee MK. Development of nested polymerase chain reaction assay for the detection and identification of Pythium insidiosum. J Vet Intern Med. 2002;16(2):147-52. http://dx.doi.org/10.1111/j.1939-1676.2002.tb02346.x. PMid:11899029.

Graham JP, Newell SM, Roberts GD, Lester NV. Ultrasonographic features of canine gastrointestinal pythiosis. Vet Radiol Ultrasound. 2000;41(3):273-7. http://dx.doi.org/10.1111/j.1740-8261.2000.tb01490.x. PMid:10850879.

Melo AL, Witter R, Martins TF, Pacheco TA, Alves AS, Chitarra CS, Dutra V, Nakazato L, Pacheco RC, Labruna MB, Aguiar DM. A survey of tick-borne pathogens in dogs and their ticks in the Pantanal biome, Brazil. Med Vet Entomol. 2016;30(1):112-6. http://dx.doi.org/10.1111/mve.12139. PMid:26467462.

Mendoza L, Newton JC. Immunology and immunotherapy of the infections caused by Pythium insidiosum. Med Mycol. 2005;43(6):477-86. http://dx.doi.org/10.1080/13693780500279882. PMid:16320491.

Nyland TG, Neelis DA, Mattoon JS. Gastrointestinal tract. In: Mattoon JS, Nyland TG, editors. Small animal diagnostic ultrasound. 3rd ed. St Louis: Saunders; 2015. 468 p. http://dx.doi.org/10.1016/B978-1-4160-4867-1.00012-X.

Trost ME, Gabriel AL, Masuda EK, Fighera RA, Irigoyen LF, Kommers GD. Aspectos clínicos, morfológicos e imunohistoquímicos da pitiose gastrintestinal canina. Pesq Vet Bras. 2009;29(8):673-9. http://dx.doi.org/10.1590/S0100-736X2009000800012.

Ubiali DG, Cruz RA, De Paula DA, Silva MC, Mendonça FS, Dutra V, Nakazato L, Colodel EM, Pescador CA. Pathology of nasal infection caused by Conidiobolus lamprauges and Pythium insidiosum in sheep. J Comp Pathol. 2013;149(2- 3):137-45. http://dx.doi.org/10.1016/j.jcpa.2012.12.002. PMid:23375916.

Downloads

Published

2022-02-25

Issue

Vol. 59 (2022)

Section

CASE REPORT

License

Copyright (c) 2022 Brazilian Journal of Veterinary Research and Animal Science

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

The journal content is authorized under the Creative Commons  BY-NC-SA  license (summary of the license: https://creativecommons.org/licenses/by-nc-sa/4.0 | full text of the license: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode) 

Funding data

How to Cite

1.
Aguiar DM de, Sifuentes M, Magalhães A, Pereira N, Pescador C, Cândido S, et al. Canine gastrointestinal pythiosis: a case report in Brazilian Pantanal from a diagnostic approach. Braz. J. Vet. Res. Anim. Sci. [Internet]. 2022 Feb. 25 [cited 2024 Apr. 25];59:e186005. Available from: https://www.revistas.usp.br/bjvras/article/view/186005