4-hydroxy-2-nonenal as a marker of the oxidative stress in brains of dogs with canine distemper


  • Érica Almeida Viscone Universidade Federal de Uberlândia, Faculdade de Medicina Veterinária https://orcid.org/0000-0001-8502-3516
  • Lígia Assunção Oliveira Universidade Federal de Uberlândia, Faculdade de Medicina Veterinária
  • Alexandra Ariadine Bittencourt Gonçalves Pereira Universidade de Brasília, Laboratório de Patologia Veterinária
  • Márcio Botelho Castro Universidade de Brasília, Laboratório de Patologia Veterinária
  • Paulo Eduardo Brandão Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal
  • Sueli Akemi Taniwaki Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal
  • Aline Santana da Hora Universidade Federal de Uberlândia, Faculdade de Medicina Veterinária
  • Márcio de Barros Bandarra Universidade Federal de Uberlândia, Faculdade de Medicina Veterinária




Demyelination, Lipid peroxidation, RT-PCR, Immunohistochemistry, 4-HNE


Canine Distemper is a disease caused by Canine morbillivirus (CM), a pantropic virus that can affect the central nervous system (CNS), causing demyelination. However, the pathogenesis of this lesion remains to be clarified. Brain samples of 14 naturally infected dogs by CM were analyzed to evaluate the presence of oxidative stress and demyelination. RT-PCR assay was performed to confirm a diagnosis of canine distemper in the brain, immunohistochemistry anti-CM was used to localize the viral proteins in the tissue, and anti-4-hydroxy-2 nonenal (4-HNE) was a marker of a product of lipid peroxidation. The results showed the presence of viral proteins in the demyelinated area with the presence of 4-HNE. Our results suggest that the CM virus infection causes oxidative stress leading to lipid peroxidation, which causes tissue damage and demyelination. In conclusion, oxidative stress plays a significant role in canine distemper pathogenesis in the CNS.


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How to Cite

Viscone, Érica A., Oliveira, L. A., Pereira, A. A. B. G., Castro, M. B. ., Brandão, P. E. ., Taniwaki, S. A. ., Hora, A. S. da, & Bandarra, M. de B. . (2022). 4-hydroxy-2-nonenal as a marker of the oxidative stress in brains of dogs with canine distemper. Brazilian Journal of Veterinary Research and Animal Science, 59, e188941. https://doi.org/10.11606/issn.1678-4456.bjvras.2022.188941




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