Pharmacopuncture with a low dosage of B complex vitamins in the Da Zhui acupoint (Governing Vessel 14) for controlling gastrointestinal parasitism in periparturient ewes

Authors

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2023.199050

Keywords:

Acupoint injection, Aquapuncture, Integrated management of parasites, Haemonchus, Periparturient egg rise

Abstract

Gastrointestinal parasites are a significant challenge in sheep farming, and periparturient ewes are one of the most susceptible categories in sheep flocks. This study aimed to verify whether the injection of B vitamins at the acupuncture point governing vessel 14 (GV14) can influence the parasitic load or host parasite interaction in naturally infected ewes during the peripartum period. Four weeks before parturition, 25 ewes were divided into five treatment groups: (1) Dose Control: 0.2 mL of B complex via IM; (2) Drug Control: 2 mL Complex B via IM; (3) Acupoint Control: 0.2 mL of Water at GV14 point; (4) Control; (5) Test group: 0.2 mL of B complex at the GV14 point. Treatments were performed at weeks -3, -1, 2, 4, and 6 about the parturition time. Fecal samples to estimate the parasitic load by fecal egg counts (FEC) and total blood samples for hemogram examination were performed seven days after the treatment, coinciding with weeks -2, 3, 5, and 7 about the parturition time. The number of peripheral eosinophils was higher in group 3 than in group 1 (p <0.05). Although groups 3 and 5 had lower mean values of FEC and higher hematological values at the time of weaning, these differences were not statistically significant (p> 0.05) according to the F test. However, all animals in groups 3 and 5 maintained low parasitism levels until the end of the experiment. Further studies should be performed with larger sample sizes and minor changes to the experimental protocol to elucidate the role of GV14 acupoint stimulation in the host-parasite relationship.

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References

Albuquerque ACA, Bassetto CC, Almeida FA, Amarante AFT. Development of Haemonchus contortus resistance in sheep under suppressive or targeted selective treatment with monepantel. Vet Parasitol. 2017;15(246):112-7. http://dx.doi.org/10.1016/j.vetpar.2017.09.010. PMid:28969773.

Amarante AFT. Importância da resposta imunológica na profilaxia da verminose ovina. In: Costa Junior L, Amarante AFT. Controle de Helmintos de Ruminantes no Brasil. 1. ed. Jundiaí: Paco Editorial; 2015. p. 203-34.

Araújo RCSA, Barbosa LP, Souza RS, Santana ALA, Carneiro IMB, Machado WM, Vieira RLA, Jesus RDL. Farmacopuntura utilizando os acupontos Hou Hai e Bai Hui em protocolos de sincronização de estro em cabras. Arq Bras Med Vet Zootec. 2019;71(02):529-37. http://dx.doi.org/10.1590/1678-4162-10333.

Bottecchia RJ. Imunomodulação pelo “point injection” no acuponto “Bai Hui” em bovinos vacinados contra brucelose e o carrapato Boophilus microplus (Canestrini, 1889) [thesis] Campos dos Goytacazes: Universidade Estadual do Norte Fluminense; 2005.

Cruvinel ACM, Vianna LR. Aplicação de substâncias em pontos de acupuntura (acuinjeção). Rev V&Z em Minas. 2017;24(132):24-31.

Estrada-Reyes Z, López-Arellano ME, Torres-Acosta F, López-Reyes A, Lagunas-Martínez A, Mendoza-de-Gives P, González-Garduño R, Olazarán-Jenkins S, Reyes-Guerrero D, Ramírez-Vargas G. Cytokine and antioxidant gene profiles from peripheral blood mononuclear cells of Pelibuey lambs after Haemonchus contortus infection. Parasite Immunol. 2017;39(6):e12427. http://dx.doi.org/10.1111/pim.12427. PMid:28345265.

Ferreira-Neto JM, Viana ES, Magalhães LM. Patologia clínica veterinária. 1. ed. Belo Horizonte: Rabelo e Brasil; 1982.

Gordon HML, Whitlock HV. A new technique for counting nematode eggs in sheep faeces. J Counc Sci Ind Res. 1939;12:50-52.

Jimenez-Sanz A, Quirino CR, Pacheco A, Costa RLD, Beltrame RT, Rua MAS, Silva RMC, Madella-Oliveira AF. Relação entre fatores associados às parasitoses gastrintestinais, desempenho e estado fisiológico das ovelhas Santa Inês. Rev Agropec Tec. 2016;37(1):88-95.

Kim SK, Bae H. Acupuncture and immune modulation. Auton Neurosci. 2010;157(1-2):38-41. http://dx.doi.org/10.1016/j.autneu.2010.03.010. PMid:20399151.

Louvandini H, Canova EB, Campos FC, Abdalla AL, McManus CM, Katiki LM, Amarante AFT. A importância da nutrição na resiliência dos ruminantes às verminoses. In: Costa Junior L, Amarante AFT. Controle de helmintos de ruminantes no Brasil. 1. ed. Jundiaí: Paco Editorial; 2015. p. 115-36.

Mendes JB, Cintra MCR, Nascimento LV, de Jesus RMM, Maia D, Ostrensky A, Teixeira VN, Sotomaior CS. Effects of protein suplementation on resistance and resilience of lambs naturally infected with gastrointestinal parasites. Semina: Ciênc Agrár. 2018;39(2):643-56. http://dx.doi.org/10.5433/1679-0359.2018v39n2p643.

National Research Council – NRC. Nutrient requirements of small ruminants: sheep, goats, cervids and new world camelids. Washington: National Academy Press; 2007.

Pons A, Canfrán S, Benito J, Cediel-Algovia R, Gómez de Segura IA. Effects of dexmedetomidine administered at acupuncture point GV20 compared to intramuscular route in dogs. J Small Anim Pract. 2017;58(1):23-8. http://dx.doi.org/10.1111/jsap.12601. PMid:27859317.

Roberts FHS, O’Sullivan PJ. Methods for egg counts and larval cultures for strongyles infesting the gastro-intestinal tract of cattle. Aust J Agric Res. 1950;1(1):99-102. http://dx.doi.org/10.1071/AR9500099.

Rosalinski-Moraes F, Fernandes FG, Munaretto A, de Oliveira S, Wilmsen MO, Pereira MW, Meirelles ACF. Famacha© system, body condition and diarrhea score as indicators for the targeted selective treatment of breeding ewes. Biosci J [Internet]. 2012 [cited 2022 Jun 12];28(6):1015-23. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/17290

Rosalinski-Moraes F, Sotomaior CS, Schmidt EMS, Thomaz Soccol V. Usage of parasitological and immunological markers for the selection of ewes resistant to gastrointestinal parasites. Arch Vet Sci. 2011;16(1):7-20. http://dx.doi.org/10.5380/avs.v16i1.19455.

Salgado JA, Santos CP. Overview of anthelmintic resistance of gastrointestinal nematodes of small ruminants in Brazil. Rev Bras Parasitol Vet. 2016;25(1):3-17. http://dx.doi.org/10.1590/S1984-29612016008. PMid:26982560.

Salgado JA, Rosalinski-Moraes F, Sotomaior CS. Endoparasitoses de pequenos ruminantes In: Sotomaior CS, Dayenoff-Rucik PM, Parraguez-Gamboa VH, Asociación Latinoamericana de Especialistas en Pequeños Rumiantes y Camélidos Sudamericanos – ALEPRyCS. Ovejas, cabras y camélidos en Lationoamérica: producción, salud y comercialización. 1. ed. Curitiba: PucPress; 2019a. p. 92-123.

Salgado JA, Cruz LV, Rocha LO, Sotomaior CS, Borges TD, Santos CP. Implication of the fecal egg count reduction test (FECRT) in sheep for better use of available drugs. Braz J Vet Parasitol. 2019b. 28(4):700-7. http://dx.doi.org/10.1590/s1984-29612019093. PMid:31800888.

Santos JP, Cavalcante RG, Souza TAA, Vieira OLE, Machado LP. Farmacopuntura com quetamina para sedação de ovinos. MV&Z CRMV-SP. 2015;12(3):74.

Santos MC, Xavier JK, Amarante MRV, Bassetto CC, Amarante AFT. Immune response to Haemonchus contortus and Haemonchus placei in sheep and its role on parasite specificity. Vet Parasitol. 2014;203(1-2):127-38. http://dx.doi.org/10.1016/j.vetpar.2014.02.048. PMid:24670867.

Scallan EM, Eckman SL, Coursey CD, Ikels KC, Simon BT. The analgesic and sedative effects of GV20 pharmacopuncture with low-dose hydromorphone in healthy dogs undergoing ovariohysterectomy. Can Vet J. 2021;62(10):1104-10. PMid:34602640.

Scognamillo-Szabó MVR, Bechara GH. Acupuntura: histórico, bases teóricas e sua aplicação em medicina veterinária. Cienc Rural. 2010;40(2):491-500. http://dx.doi.org/10.1590/S0103-84782010005000004.

Scognamillo-Szabó MVR. Efeito da acupuntura sobre a reação inflamatória imune a carrapatos Rhipicephalus sanguineus (Latreille, 18060 em cobaias (Cavia porcellus) e em cães [dissertation]. Jaboticabal: Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista “Julio de Mesquita Filho”; 1998.

Ueno H, Gonçalves PC. Manual para diagnóstico das helmintoses em ruminantes. 4. ed. Tokyo: Japan International Cooperation Agency; 1998.

van Wyk JA, Cabaret J, Michael LM. Morphological identification of nematode larvae of small ruminants and cattle simplified. Vet Parasitol. 2004;119(4):277- 306. http://dx.doi.org/10.1016/j.vetpar.2003.11.012. PMid:15154594.

Veríssimo CJ, Niciura SC, Alberti AL, Rodrigues CF, Barbosa CM, Chiebao DP, Cardoso D, da Silva GS, Pereira JR, Margatho LF, da Costa RL, Nardon RF, Ueno TE, Curci VC, Molento MB. Multidrug and multiespecies resistance in sheep flocks from São Paulo State, Brazil. Vet Parasitol. 2012;187(1-2):209-16. http://dx.doi.org/10.1016/j.vetpar.2012.01.013. PMid:22341829.

Wilmsen MO, Silva BF, Bassetto CC, Amarante AFT. Gastrointestinal nematode infections in sheep raised in Botucatu, state of São Paulo, Brazil. Braz J Vet Parasitol. 2014;23(3):348-54. https://doi.org/10.1590/S1984-29612014058.

Wu ZL, Li CR, Liu ZL, Zhang QR. Effects of acupuncture at “Zusanli” (ST36) on eosinophil apoptosis and related gene expression in rats with asthma. Zhongguo Zhen Jiu. 2012;32(8):721-5. PMid:23072095.

Xie H, Priest V. Acupuntura veterinária Xie. 1. ed. São Paulo: Editora MedVet; 2011. Yang YQ, Chen HP, Wang Y, Yin LM, Xu YD, Ran J. Considerations for use of acupuncture as supplememtal therapy for patients with allergic asthma. Clin Rev Allergy Immunol. 2013;44(3):254-61. http://dx.doi.org/10.1007/s12016-012-8321-3. PMid:22661215.

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Published

2023-01-30

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1.
Rosalinski-Moraes F, Troncha PM, Oliveira WJ, Siqueira MTS, Paiva VV de, Scognamillo MVR. Pharmacopuncture with a low dosage of B complex vitamins in the Da Zhui acupoint (Governing Vessel 14) for controlling gastrointestinal parasitism in periparturient ewes. Braz. J. Vet. Res. Anim. Sci. [Internet]. 2023 Jan. 30 [cited 2024 Jul. 23];60:e199050. Available from: https://www.revistas.usp.br/bjvras/article/view/199050