Fecal steroid and quantification in captive jaguars (Panthera onca): validation of a method

Abstract

Ovarian function of captive jaguars (Panthera onca; adults n=2 and pre-pubertal n=3) was assessed by extraction and quantification of fecal estrogens and progestins. Fecal samples were obtained 2-7 times per week during 16-18 months. Validation of solid phase radioimmunoassay for progesterone and 17b-estradiol was performed for jaguar fecal extracts. Mean concentration the fecal estrogen (ng/g of dry feces) in pre-pubertal animals was 10,97 (range, 0,28 - 59,16) and adults animals was 68,99 (range, 3,50 - 609,37). Fecal progestins (mg/g of dry feces) had a mean concentration of 0,26 (range, 0,02 - 4,44) in pre-pubertal animals and adults animals was 0,85 (range, 0,08 - 6,51).