Larval migration inhibition in tissues of mice immunized with Toxocara vitulorum antigens

Authors

  • Silvia Helena Silvestre de Paula Universidade Estadual Paulista, Faculdade de Engenharia, Departamento de Biologia e Zootecnia, Ilha Solteira, SP
  • Wilma Aparecida Starke-Buzetti Universidade Estadual Paulista, Faculdade de Engenharia, Departamento de Biologia e Zootecnia, Ilha Solteira, SP
  • Maria Francisca Neves Universidade Estadual Paulista, Faculdade de Engenharia, Departamento de Biologia e Zootecnia, Ilha Solteira, SP
  • Fabiano Pan Ferreira Universidade Estadual Paulista, Faculdade de Engenharia, Departamento de Biologia e Zootecnia, Ilha Solteira, SP

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2005.26436

Keywords:

Mus musculus, Toxocara vitulorum, Immunization, Mice

Abstract

Three groups of mice were immunized agaisnt three different Toxocara vitulorum antigens: perienteric fluid (Pe) of adults and excretory/secretory (ES) and soluble extracts (Ex) of infective larvae. A group of non-immunized animals was considered the control group. All groups were challenged one week after the third immunization with T. vitulorum infective eggs and necropsied at three different periods after the challenge: seven hours, four and 30 days. Eggs and larvae counts in the feces of mice were accomplished and revealed that Pe immunized group eliminated the highest number of larvae. Small and large intestines, liver, lungs, heart, brain and muscle (diaphragm, tongue e quadriceps femoris) were removed, digested by peptical digestion and larvae were identified and counted. The higher number of larvae was found in the large intestine seven hours after the challenge in all examined groups; however, this number was significantly lower in animals of the immunized groups. On day four after the challenge, larvae were more often found in the liver and lungs, and the immunized groups had lower numbers of larvae than in the control groups. On day 30 after the challenge low numbers of larvae were recovered in the brain and muscle. The effective immunization against larval migration based on the rate of reduction of the larvae present in the liver on day four after infection was of 82%, 79% and 58% for Ex, Pe and ES antigen, respectively.

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Published

2005-06-01

Issue

Section

UNDEFINIED

How to Cite

1.
Paula SHS de, Starke-Buzetti WA, Neves MF, Ferreira FP. Larval migration inhibition in tissues of mice immunized with Toxocara vitulorum antigens. Braz. J. Vet. Res. Anim. Sci. [Internet]. 2005 Jun. 1 [cited 2024 Jul. 24];42(3):222-30. Available from: https://www.revistas.usp.br/bjvras/article/view/26436