Cytokine detection at the site of l.(l.) Amazonensis subcutaneous inoculation in mice depleted of Natural Killer cells

Authors

  • Márcia Dalastra Laurenti Universidade de São Paulo, Faculdade de Medicina, Departamento de Patologia, Laboratório de Patologia e Doenças Infecciosas, São Paulo, SP
  • Andres Örn Karolinska Institute, Microbiology and Tumorbiology Center, Stockholm
  • Idércio Luiz Sinhorini Universidade de São Paulo, Faculdade de Medicina, Departamento de Patologia, Laboratório de Patologia e Doenças Infecciosas, São Paulo, SP
  • Carlos Eduardo Pereira Corbett Universidade de São Paulo, Faculdade de Medicina, Departamento de Patologia, Laboratório de Patologia e Doenças Infecciosas, São Paulo, SP

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2005.26440

Keywords:

Natural Killer cells, Cytokines, Cutaneous Leishmaniasis, Leishmania (Leishmania) amazonensis, Immunopathology, 90 Strontium

Abstract

BALBI c mice depleted and non-depleted of Natural Killer (NK) cells were infected subcutaneously with 107 stationary phase promastigotes of Leishmania (Leishmania) amazonensis and samples were taken at 24 hours and 7 days after infection. In NK cell-depleted mice, the NK cytotoxic activity of spleen cells decreased at 7 days after infection and more parasites were found in the lesion. The NK cell populations were analyzed by immunohistochemistry in spleen cryosections. An increase of NK1.1+ expression and a decrease of NK5E6+ antigen expression was observed in NK cell-depleted mice compared to non-depleted mice. When the presence of IFN-g, IL-12 and IL-4 at the site of parasite inoculation was analyzed by immunohistochemistry, a large amount of cytokines was detected in K cell-depleted mice at 24 hours and 7 days after infection. In non-depleted mice, there was a small amount of IL-12 at 24 hours and of IL-4 at 7 days after infection. These data cells suggest that K cell depletion by 90Srresults in increased parasitism in the lesion. The increase of NK1.1+ expression, which mainly produces IL-4, may take part in the progression of the infection.

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Published

2005-04-01

How to Cite

Laurenti, M. D., Örn, A., Sinhorini, I. L., & Corbett, C. E. P. (2005). Cytokine detection at the site of l.(l.) Amazonensis subcutaneous inoculation in mice depleted of Natural Killer cells. Brazilian Journal of Veterinary Research and Animal Science, 42(2), 105-112. https://doi.org/10.11606/issn.1678-4456.bjvras.2005.26440

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