In vitro control of prostaglandin F2a synthesis in bovine endometrium

Authors

  • Claudia Maria Bertan Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, Pirassununga, SP
  • Pauline Martins da Cunha Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, Pirassununga, SP
  • Claudio Alvarenga de Oliveira Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, Pirassununga, SP
  • Anneliese de Souza Traldi Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, Pirassununga, SP
  • Mario Binelli Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, Pirassununga, SP

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2006.26487

Keywords:

Cattle, Prostaglandin, Estradiol, Endometrium, Luteolysis

Abstract

Synthesis of endometrial prostaglandin F2± (PGF2±) results from a complex cascade of highly coordinated intracellular events. Production of PGF2± can be stimulated by mellitin and 12, 13 phorbol dibutyrate (PDBu). Objective of the present study was to verify whether basal or stimulated synthesis of PGF2± was dependent on de novo protein synthesis. In Experiment 1, endometrial explants from cyclic, non-lactating cross-bred beef cows (n=2) on day 15 of the estrous cycle were incubated in quadruplicate in culture KHB culture medium or medium supplemented with 10-6M PDBu, 10-6M melittin or 10-5M melittin. Medium samples were collected immediately and 60 minutes after treatment administration and concentration of PGF2± in medium were measured by radioimmunoassay. Concentrations of PGF2± in medium were higher (P<0.06) after treatment with PDBu in comparison with control and melittin. In Experiment 2, endometrial explants from cyclic, non-lactating cross-bred beef cows (n=4) on day 17 of the estrous cycle were incubated in quadruplicate in medium supplemented with 0, 50, 100 or 200mg cyclohexamide (CHX) and 0 or 100ng/ml PDBu, in a 4 x 2 factorial arrangement. Medium samples were collected immediately and 60 minutes after administration of treatments and concentrations of PGF2± measured by radioimmunoassay. Endometrial integrity was was evaluated by histology. Treatment with PDBu stimulated production of PGF2± (P<0.01), regardless of concentration of CHX used. The CHX did not affect production of PGF2±, either in the presence or absence of PDBu. Histological integrity of explants was preserved. It was concluded that both basal and PDBu-stimulated PGF2± production are not dependent on new protein sythesis.

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Published

2006-06-01

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UNDEFINIED

How to Cite

1.
Bertan CM, Cunha PM da, Oliveira CA de, Traldi A de S, Binelli M. In vitro control of prostaglandin F2a synthesis in bovine endometrium. Braz. J. Vet. Res. Anim. Sci. [Internet]. 2006 Jun. 1 [cited 2024 Jul. 25];43(3):387-93. Available from: https://www.revistas.usp.br/bjvras/article/view/26487