A fast immunoassay for the screening of alfa-trenbolone in bovine urine


  • Keila Maria Roncato Duarte Agência Paulista de Tecnologia dos Agronegócios, Instituto de Zootecnia e Pastagens, Nova Odessa, SP
  • Luiz Humberto Gomes Universidade de São Paulo, Escola Superior de Agricultura Luiz de Queiroz, Laboratório Laboratoio de Genetica, Piracicaba, SP
  • Jonas Augusto Rizzato Paschoal Universidade Estadual de Campinas, Laboratório de Química, Campinas, SP
  • Cyro Ferreira Meirelles BIOAGRI, Piracicaba, SP




Trenbolone, ELISA, Cattle


Brazil is one of the many countries that forbids the use of anabolic compounds, which generates difficulties on monitoring its use, once it has one of the biggest cattle herd. Therefore, several anabolic compounds are used, including trenbolone acetate. With the agreement of "Ministério da Agricultura, Pecuária e Abastecimento", an ELISA based test was done with the production of polyclonal antibodies in rabbits and testing in steers urine which received trenbolone acetate implants. The test showed to be cheap, easy and reliable to use in bovine urine to determine alfa- trenbolone, trenbolone acetate major metabolite, released in the animals urine until 60 days after implant was been injected. The results in comparison to the ones obtained by the commercial kit used by the Brazilian "Ministério da Agricultura, Pecuária e Abastecimento" were similar, with no significant differences.


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How to Cite

Duarte, K. M. R., Gomes, L. H., Paschoal, J. A. R., & Meirelles, C. F. (2006). A fast immunoassay for the screening of alfa-trenbolone in bovine urine. Brazilian Journal of Veterinary Research and Animal Science, 43(supl.), 74-79. https://doi.org/10.11606/issn.1678-4456.bjvras.2006.26538