Effect of refrigeration on the hemogasometric examination of venous blood in sheep

Authors

  • Marta Lizandra do Rêgo Leal Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Clínica Médica, São Paulo, SP
  • Pierre Castro Soares Universidade Federal Rural de Pernambuco, Recife, PE
  • Heloísa Godoi Bertagnon Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Clínica Médica, São Paulo, SP
  • Paulo Eduardo Gomes da Silva Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Clínica Médica, São Paulo, SP
  • Enrico Lippi Ortolani Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Clínica Médica, São Paulo, SP
  • Fernando José Benesi Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Clínica Médica, São Paulo, SP

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2006.26539

Keywords:

Ovine, Hemogasometry, Blood, Refrigeration

Abstract

With the objective of evaluating the effect of refrigeration on the hemogasometric exam, venous blood samples were collected from 12 healthy male sheep, Santa Ines breed, with a mean age of 4 months old, and body weight raging from 30 to 45 kg. The blood samples for the hemogasometric examination were collected in two aliquots from each animal, using dispensable needles connected to plastic syringes containing about 1000 IU sodium heparin. During and after the sampling, the care of avoiding the presence of air bubbles in the syringe was attempted. The samples without conservation were kept at room temperature, between 23 an 25ºC, and the samples under refrigeration were kept in an box containing 3 L of cold water and 3Kg of ice, to maintain a temperature between 0 and 4ºC. The hemogasometric analyses were made immediately after collection an after 1, 2, 3, 4, 5, 6, 8, 10, 12 and 24 hours. The results indicated significant alteration in samples kept at room temperature, characterized by decline, starting at 4, 8 and 10 hour post-collection, of the values f pH, BE and StB, respectively, and by a raise, since the 6th hour, of the values of PCO2. No significant variations of the parameters were seen in the refrigerated samples during the study. So, the conclusion is that ovine venous blood samples are viable, for the determination of the hemogasometric evaluation, until 24 hours after the collection, when kept (maintained) under adequate refrigeration.

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Published

2006-12-03

Issue

Section

UNDEFINIED

How to Cite

1.
Leal ML do R, Soares PC, Bertagnon HG, Silva PEG da, Ortolani EL, Benesi FJ. Effect of refrigeration on the hemogasometric examination of venous blood in sheep. Braz. J. Vet. Res. Anim. Sci. [Internet]. 2006 Dec. 3 [cited 2024 Jul. 14];43(supl.):80-5. Available from: https://www.revistas.usp.br/bjvras/article/view/26539