Use of spermatocrit to estimate sperm concentration of semen from piabanha (Brycon insignis)

Authors

  • Eduardo Shimoda Faculdade de Castelo, Castelo, ES
  • Dalcio Ricardo de Andrade Universidade Estadual do Norte Fluminense, Campos de Goytacazes, RJ
  • Manuel Vazquez Vidal Junior Universidade Estadual do Norte Fluminense, Campos de Goytacazes, RJ
  • George Shigueki Yasui Universidade de Hokkaido, Hakodate, Japão
  • Hugo Pereira Godinho Pontifícia Universidade Católica de Minas Gerais, Belo Horizonte
  • José Frederico Straggiotti Silva Universidade Estadual do Norte Fluminense, Campos de Goytacazes, RJ
  • Guilherme Sousa Universidade Estadual do Norte Fluminense, Campos de Goytacazes, RJ

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2007.26585

Keywords:

Sperm concentration, Espermatocrit, Seminal analysis, Piabanha

Abstract

The aim of this work was to determine the viability of spermatocrit use in Piabanha's sperm concentration estimation. Gametogenesis of piabanha Brycon insignis (n=29) was induced with crude pituitary extract of carp and semen was further collected. The spermatocrit was determined using cappilar tube micro-centrifuge. The sperm concentration was verified by counting in "Neubauer chamber", after dilution. The verified sperm concentration (mean±standard deviation) was 24,38±3,84x10(9) spermatozoa/mL, and the spermatocrit was 16,14±5,20%. The regression model: v = 14,01.10(9) + 0,6428.10(9).X was significant (P<0,01) and presented adjusted R² = 0,75. Thus, the estimation of sperm concentration using the spermatocrit is possible.

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Published

2007-12-03

Issue

Vol. 44 (2007): Suplemento

Section

UNDEFINIED

License

The journal content is authorized under the Creative Commons  BY-NC-SA  license (summary of the license: https://creativecommons.org/licenses/by-nc-sa/4.0 | full text of the license: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode) 

How to Cite

1.
Shimoda E, Andrade DR de, Vidal Junior MV, Yasui GS, Godinho HP, Silva JFS, et al. Use of spermatocrit to estimate sperm concentration of semen from piabanha (Brycon insignis). Braz. J. Vet. Res. Anim. Sci. [Internet]. 2007 Dec. 3 [cited 2024 Oct. 6];44(supl.):19-24. Available from: https://www.revistas.usp.br/bjvras/article/view/26585