Frozen Jaguar (Panthera onca) sperm capacitation and ability to penetrate zona free hamster oocytes


  • Regina Celia Rodrigues da Paz Universidade Federal de Mato Grosso, Faculdade de Agronomia e Medicina Veterinária, Departamento de Produção Animal, Cuiabá, MT
  • Roberta Mara Züge Instituto de Tecnologia do Paraná, Curitiba, PR
  • Valquíria Hyppólito Barnabe Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP



Panthera onca, Penetration assay, Sperm capacitation, Sperm cryopreservation


Assisted reproductive technologies in endangered species, such as artificial insemination, in vitro fertilization and embryo transfer, can be viewed as one potential approach for safeguarding species. Toward this aim, the objective of this study was to evaluate the fertility of frozen jaguar (Panthera onca) sperm and Tyrod's Talp PVA capacitation medium using the hamster zona free oocyte penetration assay. Ejaculates were collected from nine animals using electroejaculation and cryopreserved. Sperm capacitation was performed by swim-up technique using Tyrod's Talp PVA medium at room temperature. Penetration was considered when the spermatozoa head decondensation was visualized within the oocyte. This assay showed 15.4% penetrations (350/2275 oocytes). Results of this study showed high sperm abnormalities, low sperm quality after cryopreservation, and low percentage of penetrations. However, the penetration results showed that the cryopreserved jaguar's semen can be used for artificial insemination, in vitro fertilization and intra cytoplasmatic sperm injection, supporting the semen bank creation for this specie.


Download data is not yet available.




How to Cite

Paz, R. C. R. da, Züge, R. M., & Barnabe, V. H. (2007). Frozen Jaguar (Panthera onca) sperm capacitation and ability to penetrate zona free hamster oocytes. Brazilian Journal of Veterinary Research and Animal Science, 44(5), 337-344.




Most read articles by the same author(s)