Endothelin-1 effects on steroidogenesis and cellular growth of bovine granulosa cells culture

Authors

  • Luis Henrique Montrezor Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Fisiologia, Ribeirão Preto, SP
  • Alzira Amélia Martins Rosa e Silva Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Fisiologia, Ribeirão Preto, SP

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2007.26648

Keywords:

Steroidogenesis, Granulosa cells, Catte, Endothelin-1, Follicular development

Abstract

Besides the neuroendocrine control of the hypotalamic-hypophisary-gonodal axis there are autocrines and paracrines factors that control the growth, selection and ovulation in the mammals. These factors are not well established yet. Aiming to analyze the variations in the endotelin-1plasmatic concentrations in a bovine estral cycle and their effects on the culture of granulosa cells, plasma samples of 5 days (0,1,3,7 and 13) from an estral cycle have been analyzed, having as basis the ovulation day (day 0) and 3 hours (8,13,16) of the fourteenth day of the cycle. The variations in the endothelin-1 concentrations were not significant for the days as well as for the hours of the analyzed day. Granulosa cells obtained from antral follicles were cultivated during 24 hours with different concentrations of endothelin. The results demonstrated that the endothelin-1 acts in a dose-dependent manner stimulating the production of progesterone and inhibiting the cellular growth.

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Published

2007-04-01

How to Cite

Montrezor, L. H., & Rosa e Silva, A. A. M. (2007). Endothelin-1 effects on steroidogenesis and cellular growth of bovine granulosa cells culture. Brazilian Journal of Veterinary Research and Animal Science, 44(2), 103-114. https://doi.org/10.11606/issn.1678-4456.bjvras.2007.26648

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