Utilization of modified agglutination test and indirect immunofluorescent antibody test for the detection of Toxoplasma gondii antibodies in naturally exposed horses

Authors

  • Helio Langoni Universidade Estadual Paulista, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Higiene Veterinária e Saúde Pública, Núcleo de Pesquisa em Zoonoses, Botucatu, SP
  • Aristeu Vieira da Silva Universidade Paranaense, Umuarama, PR
  • Sandia Bergamaschi Pezerico Universidade Estadual Paulista, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Higiene Veterinária e Saúde Pública, Núcleo de Pesquisa em Zoonoses, Botucatu, SP
  • Vanessa Yuri de Lima Universidade Estadual Paulista, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Higiene Veterinária e Saúde Pública, Núcleo de Pesquisa em Zoonoses, Botucatu, SP

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2007.26657

Keywords:

Toxoplasma gondii, Equine, Serology, IFAT, MAT

Abstract

Infection by Toxoplasma gondii in equines is usually not apparent, it being characterized by presence of antibody titers and tissue cysts. This study was aimed at verifying the presence of anti-Toxoplasma antibodies in equine serum by modified agglutination test (MAT) and reaction to indirect immunofluorescent antibody test (IFAT). 1984 samples of serum were examined, by MAT, using whole formalin fixed tachyzoites of T.gondii as antigen. The samples reacting in the MAT test, and 150 other negative samples, chosen at random, were also tested by IFAT, utilizing anti-equine IgG. The association among the test results was verified by the McNemar test. 138 samples were positive in the MAT test, with 60 (46.38%) presenting reaction at a dilution of 1:64; 52 (37.7%) at 1:256; 19 (13.8%) at 1:1024; five (3.6%) at 1:4096; and two (1.45%) at 1:16384. Of 132 positive MAT samples, 14 were negative in the IFAT test, but the statistical analysis indicated general agreement in results of the tests. The results obtained showed agreement among the tests utilized, and the possibility of participation of equines in the transmission of toxoplasmosis to carnivorous animals, and also to humans.

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Published

2007-02-01

Issue

Section

UNDEFINIED

How to Cite

1.
Langoni H, Silva AV da, Pezerico SB, Lima VY de. Utilization of modified agglutination test and indirect immunofluorescent antibody test for the detection of Toxoplasma gondii antibodies in naturally exposed horses. Braz. J. Vet. Res. Anim. Sci. [Internet]. 2007 Feb. 1 [cited 2024 Apr. 20];44(1):27-32. Available from: https://www.revistas.usp.br/bjvras/article/view/26657