Effects of a calcium chelator, a detergent and the soybean lecithin on the quality of the frozen-thawed goat semen
Keywords:Goat, Semen, Cryopreservation, Extender
AbstractThe aim of this paper was verifying the effect of the Equex STM Paste and EDTA addition to a Tris-egg yolk extender, on the post-thaw goat sperm viability. Nine semen samples of two adult goats were collected by artificial vagina and cryopreserved. It was also objective of this study, to evaluate the utilization of a soybean lecithin based commercial extender (Bioexcell® - IMV, L'Aigle, French) for the goat semen freezing. They were formed five experimental groups: TRIS; TRIS+EDTA; TRIS+EQUEX; TRIS+EDTA+EQUEX e Bioexcell. After evaluation, the semen was diluted in the five extenders and packed in 0.25mL straws with 100 million of motile spermatozoa. The samples were cooled at 0,46°C/min to 5°C, submitted at 75min of equilibration time and frozen in liquid nitrogen vapour. The thawing was accomplished in 37ºC water bath for 50s. There were no differences (P>;0,05) on the means of post-thaw total and progressive sperm motility among the groups TRIS, TRIS+EQUEX and TRIS+EQUEX+EDTA. The Bioexcell group obtained the least (P<0,05) percentage of post-thawing total and progressive sperm motility. After the thermotolerance test, it was observed the greatest (P<0,05) rates of total and progressive sperm motility in the Equex STM groups (TRIS+EQUEX and TRIS+EQUEX+EDTA). Thus, it can be affirmed that the Equex addition promotes better maintenance rates in the pos-thaw sperm viability, when compared with the extenders that did not contain it.
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Bittencourt, R. F., Ribeiro Filho, A. de L., Lima, M. C. C., Alves, S. G. G., Vasconcelos, M. F., Biscarde, C. E., Leal, L. da S., & Oba, E. (2008). Effects of a calcium chelator, a detergent and the soybean lecithin on the quality of the frozen-thawed goat semen. Brazilian Journal of Veterinary Research and Animal Science, 45(4), 305-312. https://doi.org/10.11606/issn.1678-4456.bjvras.2008.26690
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