FSH and LH profiles during follicle deviation in Nelore heifers (Bos indicus)

Authors

  • Lindsay Unno Gimenes Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Manoel Francisco Sá Filho Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • José Ribamar Torres-Júnior Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Maria Paula Beltran Universidade Estadual Paulista, Laboratório de Endocrinologia, Departamento de Apoio e Produção em Saúde Animal, Araçatuba, SP
  • Guilherme de Paula Nogueira Universidade Estadual Paulista, Laboratório de Endocrinologia, Departamento de Apoio e Produção em Saúde Animal, Araçatuba, SP
  • Pietro Sampaio Baruselli Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2008.26714

Keywords:

Gonadotropins, Pituitary, Nelore, Radioimmunoassay, Cattle

Abstract

Present study aimed to evaluate gonadotropins profiles in 12 Nelore heifers, in order to test the hypothesis that FSH concentrations decrease and LH presents a transient increase during follicle selection. Blood samples from jugular vein were harvested twice daily starting at the time of ovulation (D0) until D5. Plasma samples were assayed for FSH and LH by double antibody radioimmunoassay method. LH and FSH assay sensitivity was 0,02ng/ml and 0,005ng/ml, respectively. The intraassay coefficient of variation was 13,6% and 18,8%, respectively. Data (mean±SEM) were normalized to follicle deviation and analyzed by ANOVA and by linear, cubic, and quadratic regressions. Comparisons between higher and lower FSH values were also performed by T-test. There was no effect of time in plasmatic FSH and LH circulating levels when variance analysis or regression analysis were performed. However, by T-test, FSH concentrations reached the lowest plasmatic levels 36 (0,40±0,05ng/ml) and 60 hours (0,42±0,04ng/ml) after follicular deviation, comparatively to 36 hours before deviation, when the concentrations were maximal (0,63±0,08ng/ml). In conclusion, there is a FSH decrease, although a transient LH elevation has not been confirmed encompassing follicle deviation in Nelore females.

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Published

2008-02-01

How to Cite

Gimenes, L. U., Sá Filho, M. F., Torres-Júnior, J. R., Beltran, M. P., Nogueira, G. de P., & Baruselli, P. S. (2008). FSH and LH profiles during follicle deviation in Nelore heifers (Bos indicus). Brazilian Journal of Veterinary Research and Animal Science, 45(1), 11-16. https://doi.org/10.11606/issn.1678-4456.bjvras.2008.26714

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