Laboratorial and physiological validation of comercial kits for quantification of fecal corticoids of captive chimpanzees (Pan troglodytes) and orangutangs (Pongo pygmaeus) under environmental enriched conditions

Authors

  • Cristiane Schilbach Pizzutto Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Manuela Gonçalves Fraga Geronymo Sgai Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Priscila Viau Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Marie Odile Monier Chelini Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Cláudio Alvarenga de Oliveira Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Marcelo Alcindo de Barros Vaz Guimarães Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP

DOI:

https://doi.org/10.11606/S1413-95962008000700015

Keywords:

Physiological validation, Biochemical validation, Fecal corticosteroids, Chimpanzee (Pan troglodytes), Orangutan (Pongo pygmaeus)

Abstract

A comparative study of fecal corticoids (FC) concentrations was carried out with chimpanzees (Pan troglodytes) e orangutans (Pongo pygmaeus). Fecal samples were collected before (Basal) and just after (Habituation) enrichment introduction and analyzed. We performed biochemical and physiological validations of the ImmunuChemTM Double Antibody Corticosterone kit for radioimmunoassay from MP Biomedicals for quantifying FC concentrations. To establish the biochemical validity of our assay we performed parallelism assays in which pooled fecal extracts from both species were spiked with known quantities of corticosterone standard and the slopes of the curves obtained with these samples and the standard curves of the kits were compared.The correlation coefficients were R^2=0.98 (Y= 17.23+1.31*X) for chimpanzees and R^2=0.99 (Y=11.14+1.29*X) for orangutans. For biological validation, we used the introduction of environmental enrichment to trigger a rise in FC levels as a consequence of the response to a stressor. Concentrations were expressed as mean and SEM. Mean concentrations of fecal corticosteroids were: Basal: 5.90 ± 2.41 x10³ ng/g of feces, Habituation: 14.92± 4.66 x10³ ng/g of feces for chimpanzees and Basal: 91.1 ± 30.0 x10³ ng/g of feces, Habituation: 185.1 ± 57 x10³ ng/g of feces for orangutans. For both species, the mean concentrations of Basal and Habituation periods were significantly different.

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Published

2008-12-01

Issue

Section

UNDEFINIED

How to Cite

1.
Pizzutto CS, Sgai MGFG, Viau P, Chelini MOM, Oliveira CA de, Guimarães MA de BV. Laboratorial and physiological validation of comercial kits for quantification of fecal corticoids of captive chimpanzees (Pan troglodytes) and orangutangs (Pongo pygmaeus) under environmental enriched conditions. Braz. J. Vet. Res. Anim. Sci. [Internet]. 2008 Dec. 1 [cited 2024 Jul. 17];45(supl.):104-10. Available from: https://www.revistas.usp.br/bjvras/article/view/26737