Electrophoresis profile of the acute phase proteins of goats experimentally infected with Trypanosoma evansi

Authors

  • Thais Helena Constantino Patelli Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterináriasl, Departamento de Clínica e Cirurgia Veterinária, Jaboticabal, SP
  • Luiz Carlos Marques Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterináriasl, Departamento de Clínica e Cirurgia Veterinária, Jaboticabal, SP
  • José Jurandir Fagliari Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterináriasl, Departamento de Clínica e Cirurgia Veterinária, Jaboticabal, SP
  • Paulo César Silva Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterináriasl, Departamento de Clínica e Cirurgia Veterinária, Jaboticabal, SP

DOI:

https://doi.org/10.11606/S1413-95962008000700020

Keywords:

Electrophoresis, Proteins, Goats, T. evansi

Abstract

Considered as one of the main agents of the tripanossomiases, Trypanosoma evansi causes a disease generically know as "surra", with wide geographic occurence. This work has the aim to study the electrophoretic profile of the acute phase proteins of goats, experimentally infected with T. evansi. Ten crossbread female goats, around 4 months of age, clinically healthy and serum negative for the presence of antibodies anti-T. evansi (IFAT) were used. The animals were divided in two groups: six were inoculated (G1) intravenously with 2,38 x 10(6) tripomastigotes of T. evansi and four were kept as non-infected controls. The blood for serum was collected daily until the 14 days after inoculation (DAI), weekly up to the 98th DAI and every two weeks up to the 364th DAI. The serum proteins were separacted by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDSPAGE). Twenty-one proteins were found in the serum of the goats, eight were nominally identified; phosphorylase, transferrin, albumin, antitrypsin, acid glicoprotein, haptoglobin, hemoglobin, and light chain immunoglobulin.

Downloads

Download data is not yet available.

Published

2008-12-01

How to Cite

Patelli, T. H. C., Marques, L. C., Fagliari, J. J., & Silva, P. C. (2008). Electrophoresis profile of the acute phase proteins of goats experimentally infected with Trypanosoma evansi. Brazilian Journal of Veterinary Research and Animal Science, 45(supl.), 141-146. https://doi.org/10.11606/S1413-95962008000700020

Issue

Section

UNDEFINIED

Most read articles by the same author(s)