Progesterone and estradiol commercial kits validation in serum of ocelots (Leopardus pardalis) and tigrina (Leopardus tigrinus)

Authors

  • Regina Celia Rodrigues da Paz Universidade Federal do Mato Grosso, Faculdade de Agronomia e Medicina Veterinária, Departamento de Ciências Básicas e Produção Animal, Cuiabá, MT
  • Cristina Harumi Adania Associação Mata Ciliar, Jundiaí, SP
  • Cláudio Alvarenga de Oliveira Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Renato Campanarut Barnabe Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2009.26772

Keywords:

Wildlife animals, Radioimmunoassay, Progesterone, Estradiol

Abstract

DPC commercial kit (Coat-A-Count - Diagnostic Products Corporation/USA) and DSL commercial kit (Diagnostic System Laboratories INC/USA) were used for progesterone and estradiol radioimmunoassay validation in serum of two endangered Brazilian felids: Leopardus pardalis (n=5) and Leopardus tigrinus (n=4) before (15-30 days) and after (24-28 hours) exogenous gonadotrophins treatment (hCG/ Novormon® and eCG/ Vetecor®; pFSH/Folltropin-V® and pLH/Lutropin-V®). Variation of estradiol concentration was 0.005 0.151 ng/ml and progesterone concentration was 0.15 - 37.22 ng/ml for Leopardus pardalis. Variation of estradiol concentration was 0.012-0.147 ng/ml and progesterone concentration was 0.15 - 37.22 ng/ml for Leopardus tigrinus. Minimum sensibility detected for progesterone was 0.004 ng/ml and for estradiol was 0.00014 ng/ml. Progesterone intra-assay coefficient was 2.58% and 6.36% and inter-assay was 0.67% and 5.55%. Estradiol intra-assay coefficient was 0.69% and 4.15%, and inter-assay was 1.40% and 3.00%. Parallelism was used for kits validation. Progesterone and DPC commercial kit Parallelism was r = 0.96 for Leopardus pardalis and r = 0.99 for Leopardus tigrinus. Estradiol and DSL commercial kit Parallelism was r = 0.98 for Leopardus pardalis and r = 0.99 for Leopardus tigrinus. In conclusion, these results showed that DPC commercial kit for serum progesterone dosages and DSL commercial kit for serum estradiol dosages can be used for Leopardus pardalis e Leopardus tigrinus. These findings are potentially valuable for the reproductive management and conservation of endangered felid populations.

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Published

2009-06-01

Issue

Section

UNDEFINIED

How to Cite

1.
Paz RCR da, Adania CH, Oliveira CA de, Barnabe RC. Progesterone and estradiol commercial kits validation in serum of ocelots (Leopardus pardalis) and tigrina (Leopardus tigrinus). Braz. J. Vet. Res. Anim. Sci. [Internet]. 2009 Jun. 1 [cited 2024 May 25];46(3):237-44. Available from: https://www.revistas.usp.br/bjvras/article/view/26772