Different Follicle-Stimulating Hormone (FSH) sources influence caprine preantral follicle viability and development in vitro

Authors

  • Deborah de Melo Magalhães Universidade Estadual do Ceará, Faculdade de Medicina Veterinária, Fortaleza, CE
  • Valdevane Rocha Araujo Universidade Estadual do Ceará, Faculdade de Medicina Veterinária, Fortaleza, CE
  • Isabel Bezerra Lima Verde Universidade Estadual do Ceará, Faculdade de Medicina Veterinária, Fortaleza, CE
  • Maria Helena Tavares de Matos Universidade Estadual do Ceará, Faculdade de Medicina Veterinária, Fortaleza, CE
  • Renata Carvalho Silva Universidade de Brasília, Departamento de Biologia Celular, Laboratório de Microscopia Eletrônica, Brasília, DF
  • Carolina Madeira Lucci Universidade de Brasília, Departamento de Biologia Celular, Laboratório de Microscopia Eletrônica, Brasília, DF
  • Sônia Nair Báo Universidade de Brasília, Departamento de Biologia Celular, Laboratório de Microscopia Eletrônica, Brasília, DF
  • Cláudio Cabral Campello Universidade Estadual do Ceará, Faculdade de Medicina Veterinária, Fortaleza, CE
  • José Ricardo de Figueiredo Universidade Estadual do Ceará, Faculdade de Medicina Veterinária, Fortaleza, CE

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2009.26787

Keywords:

Caprine, FSH, Growth, Preantral follicles

Abstract

The aim of this study was to evaluate the effects of pituitary (pFSH) or recombinant (rFSH) FSH on the survival and growth of caprine preantral follicles. Caprine ovarian tissues were in vitro cultured for one or seven days in Minimum Essential Medium (MEM) alone or containing 10, 50, 100 and 1000 ng/ml of pFSH or rFSH. Control tissues (non-cultured) and those cultured were processed for histological and ultrastructural studies. In addition, follicular and oocyte diameter were analysed. After seven days of culture, only 50 ng/ml of rFSH maintained the percentage of normal follicles similar to control. Moreover, 10 ng/ml of pFSH and all the concentrations of rFSH promoted primordial follicles activation. In addition, the presence of 50 ng/ml of rFSH promoted the highest follicular diameter at day seven of culture. In conclusion, 50 ng/ml of rFSH maintained the ultrastructural integrity of caprine preantral follicles, promoted primordial follicles activation and further growth of cultured follicles.

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Published

2009-10-01

Issue

Vol. 46 No. 5 (2009)

Section

UNDEFINIED

License

The journal content is authorized under the Creative Commons  BY-NC-SA  license (summary of the license: https://creativecommons.org/licenses/by-nc-sa/4.0 | full text of the license: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode) 

How to Cite

1.
Magalhães D de M, Araujo VR, Verde IBL, Matos MHT de, Silva RC, Lucci CM, et al. Different Follicle-Stimulating Hormone (FSH) sources influence caprine preantral follicle viability and development in vitro. Braz. J. Vet. Res. Anim. Sci. [Internet]. 2009 Oct. 1 [cited 2025 Apr. 25];46(5):378-86. Available from: https://www.revistas.usp.br/bjvras/article/view/26787