Multiple substitutions in biologically active domains of rabies virus glycoprotein can be related to pathogenic profile

Authors

  • Andrea Isabel Estévez Garcia Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP
  • Nobuyuki Mochizuki Nihon University, Nihon University Veterinary Research Center, Tóquio
  • Paulo Eduardo Brandão Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP
  • Avelino Albas Pólo Regional de Desenvolvimento Tecnológico dos Agronegócios da Alta Sorocabana, Presidente Prudente, SP
  • Fumio Honma Ito Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP

DOI:

https://doi.org/10.11606/S1413-95962011000200005

Keywords:

Bats, Glycoprotein, Pathogenicity, Phylogeny, Rabies

Abstract

Pathogenic profile of a rabies virus isolated from an insectivorous bat Lasiurus ega was compared with a rabies fixed virus strain (CVS/32) in hamster and mouse. Incubation and clinical periods, clinical manifestation and death rates were compared. Challenge of hamsters with L. ega was performed using: 10 2,611-4,021 LD50 /0,05 mL;. For CVS were used 10 3,7- 4,7 LD50 /0,05 mL. Were tested intramuscular (IM), intradermal (ID), intranasal (IN), epidermal abrasion (EA) inoculation routes. Viral antigen in brains was confirmed by Direct Immunofluorescence Test. Mortality percentages observed with L. ega rabies virus isolate were the following in hamster: 3,5 % IM, 10,710% IN; in mice: 50.0% IM, 30.0% IN. Furious rabies was predominant. Mortality percentages observed with CVS/32 in hamster: 12.5% IM, 62.5% ID, 12.5% IN; in mice 100.0% IM, 70.0% ID, 10.0% IN. Paralytic rabies was found with this strain in both animal models. Epidermic abrasion was not a suitable challenge route. Incubation period was 5-7 days for CVS and 11-16 days for L. ega isolate, meanwhile clinical periods were comprehended between 47 days for both viruses. Several substitutions were detected at antigenic domains of glycoprotein: AI (position 231), AII (3442 and 198-200), domain of fusion dependent on low pH (102179), transmembrane domain (440461) and residue 242. These viruses showed contrasting biological behaviors that can be linked to those substitutions at antigenic domains previously described.

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Published

2011-04-01

Issue

Section

UNDEFINIED

How to Cite

Multiple substitutions in biologically active domains of rabies virus glycoprotein can be related to pathogenic profile. (2011). Brazilian Journal of Veterinary Research and Animal Science, 48(2), 131-140. https://doi.org/10.11606/S1413-95962011000200005