Cervical, intradermal reaction for diagnosis of Eurytrema sp infection in cattle

Abstract

Cervical, intradermal reactions (IDR) were performed in 101, male and female, adult cattle slaughtered in the Viçosa abattoir Each animal received simultaneously and randomly 0,1ml of salina and salina extract of Eurytrema sp antigen in the following protein concentrations: 25(jg/ml, 50|jg/ml, 100pg/ml, 250Mg/ml, 500pg/ml and 1 mg/ml. Immediately after the injections, as well as 30 minutes later, the millimetered wheals
were measured by means of a millimetered ruler in order to get the mean diameter in both times (D, initial mean diameter; Di, final mean diameter). Then, Di - D, was calculated, and in the next day each pancreas was macroscopically examined in order to investigate the parasitic condition. The following conclusions were reached: 1) all IDR showing wheal increase of 2.5mm might be considered positive, whereas the other ones (with Di - D, < 2.5mm) might be regarded as negative; 2) Eurytrema sp antigen, at the moment of its use, ought to have a concentration of 250|jg/ml of protein/ml.