On the evolution of Avian infectious bronchitis virus in VERO cells

Authors

  • Paulo Eduardo Brandão Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP
  • Leonardo José Richtzenhain Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP
  • Laura Yaneth Villarreal Buitrago Coronavirus Research Group, São Paulo, SP

DOI:

https://doi.org/10.11606/issn.2318-3659.v49i5p386-390

Keywords:

IBV, Spike, Evolution, In vitro

Abstract

Avian infectious bronchitis virus (IBV) (Nidovirales: Coronaviridae) is a chicken Gammacoronavirus with the highestevolution rate in the genus and, despite the recently reported proofreading activity of its polymerase, intra and interhost diversity is a well-documented phenomenon. This study aimed to assess the genetic variation of serial passages of a variant genotype IBV strain in vitro. Strain CRG-BETA, propagated in chicken embryos, was inoculated in VERO cells monolayers up to the 4th passage and each passage was monitored with an RT-PCR targeted to the S1 gene (nt 705to 1094) and an RT-PCR to the protein 5a mRNA. All passages were positive to RT-PCRs to S1 and passages 1 to 3 to5a mRNA; S1 sequences showed no polymorphism. The finding of IBV mRNA in the cell cultures demonstrates that the CRG-BETA IBV strain is replicating in the VERO cells and regarding S1 sequence analysis, the lack of nucleotide mutations shows that CRG-BETA might have reached a fixed status. As a conclusion, different genotypes of IBV present different evolutionary patterns not only in vivo as previously known, but also in vitro, as described herein.

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Published

2012-10-26

How to Cite

Brandão, P. E., Richtzenhain, L. J., & Buitrago, L. Y. V. (2012). On the evolution of Avian infectious bronchitis virus in VERO cells. Brazilian Journal of Veterinary Research and Animal Science, 49(5), 386-390. https://doi.org/10.11606/issn.2318-3659.v49i5p386-390

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