Laboratory diagnosis of rabies by the fluorescent antibody test applied to brain tissues of experimentally inoculated mice and either preserved in formalin or under refrigeration

Authors

  • Marcia Ester Parreira Vasconcellos Prefeitura de Santo André, SP
  • Silvio Arruda Vasconcelos Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, São Paulo, SP
  • José de Angelis Côrtes Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, São Paulo, SP

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.1993.52012

Keywords:

Rabies (diagnosis), Fluorescent Antibody Technique, Trypsin, Pepsin

Abstract

The fitness of formalin - treated materials for use in rapid rabies diagnosis was evaluated through Fluorescent Antibody (FA) test, using the digestion technique of pepsin and trypsin and the impression method of slide preparation. To achievc this proposal, brain fragments of experimentally rabid mice were submitted to different treatments of brain preservation by either using. pH adjusted formalin solutions or refrigeration, and the FA tests were run at ten experimental phases for a test period of 28 days. The results of FA reactivity ranged from 90.0% to 58.0%, depending on the treatments submitted; under the condition of the experiment, brain specimens should be maintained at refrigerating temperature until 96 hours for suitable FA examination, over this period the results should be irregular due to tissue degradation. The preservation of brain fragments in formalin, with further enzymatic digestion of pepsin and trypsin and the use of impression method did not mask or alter the virus antigenicity for adequate identification through FA technique, although the procedures should never substitute the existing methods of rapid rabies diagnosis.

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Published

1993-06-01

How to Cite

Vasconcellos, M. E. P., Vasconcelos, S. A., & Côrtes, J. de A. (1993). Laboratory diagnosis of rabies by the fluorescent antibody test applied to brain tissues of experimentally inoculated mice and either preserved in formalin or under refrigeration. Brazilian Journal of Veterinary Research and Animal Science, 30(1), 21-24. https://doi.org/10.11606/issn.1678-4456.bjvras.1993.52012

Issue

Section

PREVENTIVE VETERINARY MEDICINE

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