Influence of organic soil on the mycoliactericidal activity of the commercial sodium hypochlorite with 2.5% of active chlorine

Authors

  • Andrea Castillo Guerrero Ministério da Agricultura, República Dominicana
  • Sonia Regina Pinheiro Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, São Paulo, SP
  • Zenáide Maria de Morais Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, São Paulo, SP
  • Silvio Arruda Vasconcellos Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, São Paulo, SP
  • Eumio Honma Ito Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, São Paulo, SP
  • José Soares Ferreira Neto Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, São Paulo, SP

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.1993.52034

Keywords:

Desinfection, Mycobacteria, Sodium hypochlorite

Abstract

It was evaluated the highest working dilution of the sodium hypochlorite solution (available as household and laundry bleach, with 2.5% of active chlorine) still effective in inactivating Mycobacterium fortuitum in the presence of different levels of bovine feces as organic soil. The experiments were conducted "in vitro" using filter paper as carriers. The time of contact was one hour at the temperature range of 4 to 8°C. The blocking of the desinfectant activity was achieved by the dilution using a solution of 0.5% sodium thiosulphate with 0.05% twenn-80 (v/v). The remaining microrganisms were cultivated in Lowenstcin-Jensen culture medium and the number of colonies forming unities was statiscally evaluated by the MannWhitneyTest. The results showed that the organic soil affected the desinfectant activity. The working dilutions calculated were 1:64 and 1:16, respectively, with and without organic soil. In working dilutions higher than 1:64 the regularity of the desinfectant action was very poor.

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Published

1993-12-03

Issue

Section

PREVENTIVE VETERINARY MEDICINE

How to Cite

1.
Guerrero AC, Pinheiro SR, Morais ZM de, Vasconcellos SA, Ito EH, Ferreira Neto JS. Influence of organic soil on the mycoliactericidal activity of the commercial sodium hypochlorite with 2.5% of active chlorine. Braz. J. Vet. Res. Anim. Sci. [Internet]. 1993 Dec. 3 [cited 2024 Jul. 17];30(supl.):211-6. Available from: https://www.revistas.usp.br/bjvras/article/view/52034