Study of embryos colect technique, frozen and thawing of Saanen (Capra hircus), goats bearing 5/15 translocation

Authors

  • Nereu Carlos Prestes Universidade Estadual Paulista, Faculdade de Medicina Veterinária e Zootecnia, Botucatu, SP
  • Cezinande de Meira Universidade Estadual Paulista, Faculdade de Medicina Veterinária e Zootecnia, Botucatu, SP
  • Carlos Antonio de Miranda Bonfim Universidade Estadual Paulista, Faculdade de Medicina Veterinária e Zootecnia, Botucatu, SP
  • Sony Dinias Bicudo Universidade Estadual Paulista, Faculdade de Medicina Veterinária e Zootecnia, Botucatu, SP
  • Maria Denise Lopes Universidade Estadual Paulista, Faculdade de Medicina Veterinária e Zootecnia, Botucatu, SP
  • Frederico Ozanam Papa Universidade Estadual Paulista, Faculdade de Medicina Veterinária e Zootecnia, Botucatu, SP

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.1993.52040

Keywords:

Embryos, Cryopreservation, Thawing, Goats

Abstract

This work studied the surgical technique for collecting embryos in the goat, using heterozygous donors for the 5/15 translocation, which were bred with translocated animals. At the same, time technical characteristics related to cryopreservation, thawing and cultivation of the structures were performed. Considering the superovulatory treatment, oestrus was observer 24 or 48 hours after removing the intravaginal sponge and 71 viable structures were yielded in 43 collections. The 1 -2 Propanediol proved to be an efficient cryoprotectant agent, and allowed the cultivation of all the embryos. This result was not observed when glycerol was used as a cryoprotectant agent. Adhesions due to multiple collections constitute a great challenge for the improvement of this technique, since they are considered a limiting factor to the use of donors in the beginning of their reproductive lives.

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Published

1993-12-03

How to Cite

Prestes, N. C., Meira, C. de, Bonfim, C. A. de M., Bicudo, S. D., Lopes, M. D., & Papa, F. O. (1993). Study of embryos colect technique, frozen and thawing of Saanen (Capra hircus), goats bearing 5/15 translocation. Brazilian Journal of Veterinary Research and Animal Science, 30(supl.), 249-254. https://doi.org/10.11606/issn.1678-4456.bjvras.1993.52040

Issue

Section

ANIMAL REPRODUCTION

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