Interaction of bovine alpha2 macroglobulin with proteinases
DOI:
https://doi.org/10.11606/issn.1678-4456.bjvras.1994.52053Keywords:
Cattle, Plasma, Proteinase inhibitors, Alpha2M, Plasmin, TrypsinAbstract
The alpha2-macroglobulin (alpha2M) protease inhibitor was purified from bovine plasma. The alpha2M preparations at various purification steps were identified by immunodiffusion and crossed immunoelectrophoresis with anti-human alpha2 serum. Anti-bovine alpha2M serum was prepared for the quantitative determinations. The purest alpha2M preparation was obtained by affinity chromatography and used as primary standard in radial immunodiffusion. Alpha2M preparations were submitted to binding tests with p' - NPGB (p‘ - nitrophenyl-p-guanidinebenzoate HCL) titrated trypsin and plasmin. Alpha2M protected 35% of the esterolytic activity of trypsin and 50% of the amidolytic activity of plasmin.
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