Evaluation of an oil emulsified vaccine against bovine colibacillosis using semi-purified K99-F41 adhesins

  • Marcina Garcia Universidade Estadual de Campinas, Campinas, SP.
  • Domingos da Silva Leite Universidade Estadual de Campinas, Instituto de Biologia, Campinas, SP.
  • Tomomasa Yano Universidade Estadual de Campinas, Instituto de Biologia, Campinas, SP.
  • Antonio Fernando Pestana de Castro Universidade Estadual de Campinas, Instituto de Biologia, Campinas, SP.
  • Maria Aparecida Moreira Shenk Empresa Brasileira de Pesquisa Agropecuária, Centro Nacional de Gado de Corte, Campo Grande, MS.
Keywords: Escherichia coli, Bovidae, Vaccines

Abstract

The K99 and F41 fimbrial antigens were extracted from Escherichia coli B41 strain by heating, and semi-purified by precipitation with ammonium sulfate and treatment with sodium desoxicolate (DOC). The semi-purified antigens K99 and F4I were used to prepare an oil emulsified vaccine against bovine colibacillosis. The vaccine was made up containing in each dose 1,500, 750 or 380 HU (Haemagglutinating Units). The test of the vaccine efficiency was measured by gel immunodiffusion and ELISA test. Challenge of calves from vaccinated and control cows was carried out with the virulent Escherichia coli B4I. Our findings suggested that 750 HU vaccine, induces better production of anti-K99-F4l antibodies in vaccinated cows, and the transference of these antibodies to calves through the colostrum, gave full protection to challenge. Furthermore no adverse effects were observed in any of the vaccinated cows.

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Published
1994-12-03
How to Cite
Garcia, M., Leite, D., Yano, T., Castro, A. F., & Shenk, M. A. (1994). Evaluation of an oil emulsified vaccine against bovine colibacillosis using semi-purified K99-F41 adhesins. Brazilian Journal of Veterinary Research and Animal Science, 31(3-4), 225-232. https://doi.org/10.11606/issn.1678-4456.bjvras.1994.52070
Section
PREVENTIVE VETERINARY MEDICINE