Study of the immunopathogenesis of central nervous system lesions in dogs naturally affected by visceral leishmaniasis

Authors

  • Tatianna Frate Schwardt Universidade Estadual Paulista, Faculdade de Medicina Veterinária, Araçatuba, SP
  • Juliana Peloi Vides Universidade Estadual Paulista, Faculdade de Medicina Veterinária, Araçatuba, SP
  • Eveline Tozzi Braga Universidade Estadual Paulista, Faculdade de Medicina Veterinária, Araçatuba, SP
  • Acácio Duarte Pacheco Universidade Estadual Paulista, Faculdade de Medicina Veterinária, Araçatuba, SP
  • Hélio Langoni Universidade Estadual Paulista, Faculdade de Medicina Veterinária, Botucatu, SP
  • Diego Generoso Universidade Estadual Paulista, Faculdade de Medicina Veterinária, Botucatu, SP
  • Gisele Fabrino Machado Universidade Estadual Paulista, Faculdade de Medicina Veterinária, Araçatuba, SP
  • Márcia Dalastra Laurenti Universidade de São Paulo, Faculdade de Medicina, Departamento de Patologia, São Paulo, SP
  • Mary Marcondes Universidade Estadual Paulista, Faculdade de Medicina Veterinária, Araçatuba, SP

DOI:

https://doi.org/10.11606/issn.1678-4456.v49i6p442-451

Keywords:

TCD3 , TCD4 , TCD8 , Macrophages, Leishmania (Leishmania) infantum chagasi

Abstract

Visceral leishmaniasis in dogs is described as a chronic disease whose main symptoms are progressive weigth loss, cachexy and dermatologic lesions. Recently, the disease has been associated to neurologic disorders. A total of 40 dogs with visceral leishmaniasis were divided into two groups. The first composed of dogs without neurological signs (n=30) and the second by dogs with neurological disorders (n=10). Brain samples were collected, stored in 10% buffered formalin and subjected to immunohistochemical examination for amastigotes forms of Leishmania (Leishmania) infantum chagasi, CD3+, CD4+ and CD8+ T lymphocytes and macrophages. Imunnohistochemistry evaluation revealed no amastigote forms of the parasite. CD3+ T lymphocytes were present in 24/30 (80%) dogs without neurological signs and in all dogs from the second group (p=0.0011). CD4+ and CD8+ were rarely observed, with CD4+ immunostaining in 10/40 (25%) dogs, from which half of them had neurological disease (p=0.0090). The presence of CD8+ was detected only in 4/10 (40%) dogs from neurological group (p=0.0021). Macrophages were detected in 38/40 (95%) dogs, without significant differences between groups (p=0.7664).

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Published

2012-12-11

How to Cite

Schwardt, T. F., Vides, J. P., Braga, E. T., Pacheco, A. D., Langoni, H., Generoso, D., Machado, G. F., Laurenti, M. D., & Marcondes, M. (2012). Study of the immunopathogenesis of central nervous system lesions in dogs naturally affected by visceral leishmaniasis. Brazilian Journal of Veterinary Research and Animal Science, 49(6), 442-451. https://doi.org/10.11606/issn.1678-4456.v49i6p442-451

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