Evaluation of extraction methods for progesterone determination in rabbit (Oryctolagus cuniculus) feces by radioimmunoassay

Authors

  • Clotilde Maria Korndörfer Centro de Energia Nuclear na Agricultura, Seção de Ciências Animais, Piracicaba, SP
  • Cyro Ferreira Meirelles Centro de Energia Nuclear na Agricultura, Seção de Ciências Animais, Piracicaba, SP
  • Ives Cláudio da Silva Bueno Centro de Energia Nuclear na Agricultura, Seção de Ciências Animais, Piracicaba, SP
  • Adibe Luiz Abdalla Centro de Energia Nuclear na Agricultura, Seção de Ciências Animais, Piracicaba, SP

DOI:

https://doi.org/10.1590/S1413-95961998000300004

Keywords:

Progesterone, Feces, Radioimmunoassay, Rabbit

Abstract

The purpose of this study was to find a practical procedure for the extraction of progesterone (P4) from feces and to determine if the P4 plasma profiles during pregnancy were reflected in the total fecal P4 of pregnant rabbits. The rabbit was used as model for the techniques. Plasma and feces were collected from 11 rabbits during a period of 42 days. Three different methods of P4 extraction were used. The total P4 was measured by solid-phase radioimmunoassay (RIA) with 125I-P4 as the tracer. Results suggested that it was possible to extract total P4 from rabbit feces with methanol and petroleum ether. Plasma and fecal P4 profiles were compared for both pregnant and ovariectomized rabbits. It was possible to differentiate total P4 extracted from day two through 28 after breeding (P<0.01).

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Published

1998-01-01

How to Cite

Korndörfer, C. M., Meirelles, C. F., Bueno, I. C. da S., & Abdalla, A. L. (1998). Evaluation of extraction methods for progesterone determination in rabbit (Oryctolagus cuniculus) feces by radioimmunoassay. Brazilian Journal of Veterinary Research and Animal Science, 35(3), 115-119. https://doi.org/10.1590/S1413-95961998000300004

Issue

Vol. 35 No. 3 (1998)

Section

VETERINARY MEDICINE

License

The journal content is authorized under the Creative Commons  BY-NC-SA  license (summary of the license: https://creativecommons.org/licenses/by-nc-sa/4.0 | full text of the license: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode)