Evaluation of extraction methods for progesterone determination in rabbit (Oryctolagus cuniculus) feces by radioimmunoassay
DOI:
https://doi.org/10.1590/S1413-95961998000300004Keywords:
Progesterone, Feces, Radioimmunoassay, RabbitAbstract
The purpose of this study was to find a practical procedure for the extraction of progesterone (P4) from feces and to determine if the P4 plasma profiles during pregnancy were reflected in the total fecal P4 of pregnant rabbits. The rabbit was used as model for the techniques. Plasma and feces were collected from 11 rabbits during a period of 42 days. Three different methods of P4 extraction were used. The total P4 was measured by solid-phase radioimmunoassay (RIA) with 125I-P4 as the tracer. Results suggested that it was possible to extract total P4 from rabbit feces with methanol and petroleum ether. Plasma and fecal P4 profiles were compared for both pregnant and ovariectomized rabbits. It was possible to differentiate total P4 extracted from day two through 28 after breeding (P<0.01).Downloads
Download data is not yet available.
Downloads
Published
1998-01-01
How to Cite
Korndörfer, C. M., Meirelles, C. F., Bueno, I. C. da S., & Abdalla, A. L. (1998). Evaluation of extraction methods for progesterone determination in rabbit (Oryctolagus cuniculus) feces by radioimmunoassay. Brazilian Journal of Veterinary Research and Animal Science, 35(3), 115-119. https://doi.org/10.1590/S1413-95961998000300004
Issue
Section
VETERINARY MEDICINE
License
The journal content is authorized under the Creative Commons BY-NC-SA license (summary of the license: https://