Comparison of five extenders for canine semen freezing

Authors

  • Silvia Edelweiss Crusco dos Santos Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Camila Infantosi Vannuchi Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Sandra Satzinger Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Mayra Elena Ortiz D’Avila Assumpção Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • José Antonio Visintin Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP

DOI:

https://doi.org/10.1590/S1413-95961999000500004

Keywords:

Diluents, Freezing, Semen, Dogs

Abstract

Semen was collected from six stud dogs to compare five extenders in the semen freezing process. Each ejaculate was divided in five parts and added to tris-fructose-citric acid, glicine, lactose, skim milk and tris-fructose-citrate extenders. The semen was diluted at 37°C in extenders without glycerol, in the ratio 1:1 and cooled for 60 minutes to reach a 5°C temperature. Then, extenders with glycerol in the ratio of 2:1 were added to give the final prefreezing concentration of 4% of glycerol. The diluted semen with the cryoprotectant was maintained for a further 60 minutes in refrigeration to equilibrate the spermatozoa in the glycerol and packaged in 0.5 ml plastic straws. The straws were maintained for 30 minutes in vapor, plunged and stored in liquid nitrogen. Sperm morphology was evaluated before and after freezing, whereas progressive motility (%) and velocity of forward progression (0-5) were appraised in different periods of the freezing process. The extender tris-fructose-citric acid showed the best post thaw progressive motility and velocity of forward progression compared to the others extenders. Semen freezing increased major sperm morphological abnormalities, regardless of the extender.

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Published

1999-01-01

Issue

Section

VETERINARY MEDICINE

How to Cite

1.
Santos SEC dos, Vannuchi CI, Satzinger S, Assumpção MEOD, Visintin JA. Comparison of five extenders for canine semen freezing. Braz. J. Vet. Res. Anim. Sci. [Internet]. 1999 Jan. 1 [cited 2024 Jul. 19];36(5):244-9. Available from: https://www.revistas.usp.br/bjvras/article/view/5735