Superovulation of Nelore heifers with different FSH/LH doses and embryo freezing by one-step method with ethylene-glycol
Keywords:Superovulation, Embryos, Ethylene-glycol, Cattle
AbstractThe objetive of this study was to identify the better dose between 300 (n = 20), 400 (n = 21) and 500 IU (n = 21) of FSH/LH to stimulate Nelore heifers. The superovulation treatment started on day 10 (D0 = estrous) of the estrous cycle in 8 decreasing aplications for 4 days. The embryo recovery was achieved on day 6.5 after the first artificial insemination. The superovulatory response for 300, 400 and 500 IU FSH/LH was follicles (15.12, 15.76 and 14.94); corpus luteum (10.68, 11.55 and 10.81) and transferable embryos (5.20, 1.81 and 2.76). The 300 IU of FSH/LH group presented the best results in regard to transferable embryos. The transferable embryos were cryopreserved by one-step method with 1.5 M of ethylene-glycol, resulting in 8 pregnancies (7.5%) of 106 embryos transferred by non-curgical method. The 300 IU of FSH/LH presented better superovulatory response in comparison with 400 and 500 IU in Nelore heifers. The transfer of Bos taurus indicus embryos cryopreserved by one-step method in 1.5 M of ethilene glycol was not efficient.
Download data is not yet available.
How to Cite
Visintin, J. A., Arruda, R. P. de, Madureira, E. H., Mizuta, K., Celeghini, E. C. C., Assumpção, M. E. O. D., Gusmões, P. P. G., & Candini, P. H. (1999). Superovulation of Nelore heifers with different FSH/LH doses and embryo freezing by one-step method with ethylene-glycol. Brazilian Journal of Veterinary Research and Animal Science, 36(5), 267-272. https://doi.org/10.1590/S1413-95961999000500009
The journal content is authorized under the Creative Commons BY-NC-SA license (summary of the license: https://