Diagnostic efficiency of Brucella soluble antigens in immunodiffusion tests and ability to differentiate Brucella abortus strain 19 vaccinated cattle
Keywords:rucellosis, Immunodiffusion tests, Vaccination, Cattle
AbstractThree soluble antigens were compared by radial immunodiffusion (RID) and agar gel immunodiffusion (AGID) tests: a native haptene (NH) from Brucella melitensis 16M, and a polysaccharide (PS) from B. abortus 1119-3, both obtained by non-hydrolytic methods, and the (O-Chain) polysaccharide extracted also from B. abortus 1119-3 but using an hydrolytic method. Three groups of bovine sera were tested: a) Naturally infected (n = 76); b) Non-infected (n = 130) and c) S-19 vaccinated (n = 61); the sensitivity (Se), the specificity (Sp) and the ability to differentiate vaccinated (ADV) were determined in each group a, b and c respectively. The highest Se in the RID test (84.3%) was achieved by NH; while the three antigens gave 100% Sp. The O-Chain showed 100% ADV in this test. In the AGID test PS antigen showed the best Se (86.6%), and all antigens showed 100% of Sp and ADV. Finally, for its production qualities and efficiency the antigens PS and NH represent a promising alternative for complementary diagnosis of brucellosis.
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How to Cite
Daffner, J., Abalos, P., Pinochet, L., Scortti, M., & Urcelay, S. (1999). Diagnostic efficiency of Brucella soluble antigens in immunodiffusion tests and ability to differentiate Brucella abortus strain 19 vaccinated cattle. Brazilian Journal of Veterinary Research and Animal Science, 36(3), 116-120. https://doi.org/10.1590/S1413-95961999000300002
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