Penetration assay of frozen jaguar (Panthera onca) sperm in heterologous oocytes

Authors

  • Regina Celia Rodrigues da Paz Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Roberta Mara Züge Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Valquíria Hyppolito Barnabe Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Ronaldo Gonçalves Morato Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Paulo Anselmo Nunes Felippe Bosque dos Jequitibás, Campinas, SP
  • Renato Campanarut Barnabe Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP

DOI:

https://doi.org/10.1590/S1413-95962000000600008

Keywords:

Panthera onca, Semen, Criopreservation, Sperm capacitation, Trials, Penetration, Oocytes

Abstract

Assisted reproductive technologies can be viewed as one potential approach for safeguarding wild species. In this study were evaluated the fertility of captivity male jaguar (Panthera onca) and different capacitation media using the golden hamster zona free oocyte penetration assay. We used frozen/thawed semen from 3 animals housed at Bosque dos Jequitibás, Campinas/SP, to test the Percoll gradient, Swim-up and Swim-up + 1 h incubation (5% CO2 / 38°C), considering as penetration the spermatozoa head descondensation visualized within the oocyte. The results rate was greater for Percoll (26.5%) as compared to Swim-up (8.1%) (X2 = 19.93; p < 0.05). It was not observed penetration with Swim-up + 1 h incubation (5% CO2 / 38°C). It is concluded that Percoll and Swim-up are efficient methods to perform the golden hamster zona free oocyte penetration assay using frozen/thawed jaguar (Panthera onca) spermatozoa. The low rate of penetration could be related to the high rate of morphological abnormal spermatozoa observed in the samples examined.

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Published

2000-12-01

How to Cite

Paz, R. C. R. da, Züge, R. M., Barnabe, V. H., Morato, R. G., Felippe, P. A. N., & Barnabe, R. C. (2000). Penetration assay of frozen jaguar (Panthera onca) sperm in heterologous oocytes. Brazilian Journal of Veterinary Research and Animal Science, 37(6), 462-466. https://doi.org/10.1590/S1413-95962000000600008

Issue

Section

VETERINARY MEDICINE

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