Comparison of a commercial enzyme immune assay (ELISA) and serum neutralization techniques for the detection of antibodies against BHV-1 in buffaloes (Bubalus bubalis) sera
Keywords:Infections bovine rhinotracheites, ELISA, Buffaloes, Bubalus bubalis
AbstractThe performance of a commercial ELISA (Bovine Rhinotracheitis virus antibody test Kit, Herdcheck®, IDEXX Laboratories Inc., USA) designed to detect the presence of antibody to the Bovine Herpesvirus type 1 (BHV-1) in bovine serum, was evaluated in samples of buffaloes (Bubalus bubalis) serum. Sera of 133 buffaloes without IBR vaccination were performed both by ELISA and standard serum neutralization technique. Sensitivity and specificity of ELISA were 97.14% and 46.03%, respectively. The estimated concordance between two serologic diagnostic methods by kappa index was 0.44 IC (0.29-0.59) using 95% confidence level.
How to Cite
The journal content is authorized under the Creative Commons BY-NC-SA license (summary of the license: https://