Evaluation of in vitro sperm capacitation with heparin and calcium ionophore in bulls

Authors

  • Mayra Elena Ortiz D'Avila Assumpção Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Kátia Haipeck Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Alecsandra Sobreira de Lima Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Marco Roberto Bourg de Mello Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Lilian Jesus de Oliveira Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Viviane Purri de Oliveira Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Liliam Mara Trevisan Tavares Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • José Antônio Visintin Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP

DOI:

https://doi.org/10.1590/S1413-95962002000300008

Keywords:

Sperm capacitation, Heparin, Calcium ionophore, Staining methods

Abstract

The aim of this particular study was to test in vitro sperm capacitation protocols, using heparin (100mg/ml) and calcium ionophore (5mM). Propidium iodide and carboxifluorescein diacetate (IP/CFDA) in a fluorescence microscope as well as triple stain (congo red, neutral red and Giemsa) in Phase contrast microscope were used as staining. The spermatozoa were classified according to its viability (alive or dead) and acrossome integrity (damaged or intact). They were considered as follows: capacitated (alive and damaged); non capacitated (alive and not damaged) and dead (damaged or intact). The heparin group showed a ratio of 64.54% and 39.16% of capacitated spermatozoa in IP/CFDA and triple stain, respectively. In the calcium group, 36.41% and 18.11% of spermatozoa were capacitated in IP/CFDA and triple stain, respectively. Bulls were divided into 3 groups according to their fertility rates as follows: Group A < 63%, Group B from 63 to 68% and Group C >; 68%. For all three groups there was significant differences (p;0.01), when observed capacitated, non capacitated and dead spermatozoa among groups A and B; A and C; B and C, using heparin and calcium ionophore in both stains. No correlation was seen between capacitation and fertility rates. Therefore heparin treatment showed better sperm capacitation rates than calcium ionophore. The IP/CFDA technique showed itself as being a better method to evaluate sperm capacitation than the triple stain (p<0.01).

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Published

2002-01-01

Issue

Section

UNDEFINIED

How to Cite

1.
Assumpção MEOD, Haipeck K, Lima AS de, Mello MRB de, Oliveira LJ de, Oliveira VP de, et al. Evaluation of in vitro sperm capacitation with heparin and calcium ionophore in bulls. Braz. J. Vet. Res. Anim. Sci. [Internet]. 2002 Jan. 1 [cited 2024 Jul. 25];39(3):149-56. Available from: https://www.revistas.usp.br/bjvras/article/view/5978