Histological changes in Lymnaea columella caused by the Euphorbia splendens's latex
Keywords:Fasciola hepatica, Euphorbia splendens, Lymnaea columella, Control
AbstractHistological observations in Lymnaea columella snails to determine the existence or not of changes caused by the use of the Euphorbia splendens's latex were realized. The experiment was realized in agreement with the methods standardized by WHO. Fifty samples of L. columella, with shell 6±1mm long, were submitted at 200ml of the solutions in concentrations of 0,47µl/L; 0,76µl/L; 0,94µl/L and 1,51µl/L, during 24 hours. After the experimental event, the snails were placed in solution of Raillet & Henry, for sacrifice and fixation. The results demonstrated lesions characterized by degeneration, necrosis and liquid accumulation in the digestive gland and in the kidney, in samples maintained in concentrations from 0,47 mul/L on.
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