The influence of the route of inoculation on the development of the leptospiral infection in hamsters (Mesocricetus auratus) experimentally infected with Leptospira interrogans serovar pomona

Authors

  • Nicodemos Alves de Macedo Universidade Federal do Piauí, Centro de Ciências Agrárias, Teresina, PI
  • Zenáide Maria de Morais Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP
  • Cláudio Roberto de Almeida Camargo Secretaria de Saúde do Estado de São Paulo, Instituto Butantã, São Paulo, SP
  • Clebert José Alves Universidade Federal de Campina Grande, Centro de Saúde e Tecnologia Rural, Departamento de Medicina Veterinária, Campina Grande, PB
  • Sérgio Santos de Azevedo Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP
  • Rodolfo Nümberger Júnior Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Patologia, São Paulo, SP
  • Sílvio Arruda Vasconcellos Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP

DOI:

https://doi.org/10.1590/S1413-95962004000300008

Keywords:

Rout of inoculation, Hamsters, Serovar pomona

Abstract

The influence of the route of inoculation on the development of the leptospiral infection was investigated in 60 hamsters (Mesocricetus auratos) experimentally infected with Leptospira interrogans serovar pomona. The routes of inoculation were intraperitoneal, subcutaneous, oral, conjuntival and scrapped skin. All animals received the leptospiral inoculum cultivated in Fletcher's medium, with 20 to 30 leptospires per field under 200x magnification. The controls were inoculated only with Fletcher's medium. Samples of blood sera and the kidney fragments were collected in the agonic stage of the disease or at 21st experimental day, when all survivors were killed. The antibodies detection was done by the microscopic agglutination test and the bacteria detection by the dark field microscopic examination and culture in Fletcher's medium, by the tenfold dilutions technique. The presence and development of disease was affected by the route of infection. The oral route was the less effective for promoting the disease. There was no association between the frequency of carrier state and the route of infection.

Downloads

Download data is not yet available.

Published

2004-06-01

Issue

Section

UNDEFINIED

How to Cite

The influence of the route of inoculation on the development of the leptospiral infection in hamsters (Mesocricetus auratus) experimentally infected with Leptospira interrogans serovar pomona. (2004). Brazilian Journal of Veterinary Research and Animal Science, 41(3), 194-200. https://doi.org/10.1590/S1413-95962004000300008