In vitro evaluation of oocyte nuclear maturation in young and adult domestic cats (Felis catus)

Authors

  • Maria Denise Lopes Universidade Estadual Paulista, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal e Radiologia Veterinária, Botucatu, SP
  • Edilson Uiechi Universidade Estadual Paulista, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal e Radiologia Veterinária, Botucatu, SP
  • Luzia A. Trinca Universidade Estadual Paulista, Instituto de Biociências, Departamento de Bioestatística, Botucatu, SP

DOI:

https://doi.org/10.1590/S1413-95962004000400008

Keywords:

Domestic cat, Follicles, Nuclear maturation

Abstract

This study assessed the in vitro oocyte nuclear maturation in adult and young domestic cats. Fifteen ovaries were used; 10 from adult females and 5 from young females. The oocytes collected by aspiration were quantified and classified. The oocytes classified as excellent and/or regular were grouped (10 oocyte /drop) in culture medium covered with mineral oil in disposable Petri dishes. The oocyte were incubated at 38°C and 5% de CO2 for 48 hours and then washed with 0,4% hyaluronidase, fixed in methanol/acetic acid and stained with acetic orcein. Evaluation of chromosomal configuration of oocytes matured in vitro showed 44,68% of oocyte in metaphase II in the adult female group and 25,32% in the young donor group, showing that puberty influences oocyte capacity for in vitro development.

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Published

2004-08-01

How to Cite

Lopes, M. D., Uiechi, E., & Trinca, L. A. (2004). In vitro evaluation of oocyte nuclear maturation in young and adult domestic cats (Felis catus). Brazilian Journal of Veterinary Research and Animal Science, 41(4), 268-273. https://doi.org/10.1590/S1413-95962004000400008

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