In vitro evaluation of oocyte nuclear maturation in young and adult domestic cats (Felis catus)
DOI:
https://doi.org/10.1590/S1413-95962004000400008Keywords:
Domestic cat, Follicles, Nuclear maturationAbstract
This study assessed the in vitro oocyte nuclear maturation in adult and young domestic cats. Fifteen ovaries were used; 10 from adult females and 5 from young females. The oocytes collected by aspiration were quantified and classified. The oocytes classified as excellent and/or regular were grouped (10 oocyte /drop) in culture medium covered with mineral oil in disposable Petri dishes. The oocyte were incubated at 38°C and 5% de CO2 for 48 hours and then washed with 0,4% hyaluronidase, fixed in methanol/acetic acid and stained with acetic orcein. Evaluation of chromosomal configuration of oocytes matured in vitro showed 44,68% of oocyte in metaphase II in the adult female group and 25,32% in the young donor group, showing that puberty influences oocyte capacity for in vitro development.Downloads
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Published
2004-08-01
How to Cite
Lopes, M. D., Uiechi, E., & Trinca, L. A. (2004). In vitro evaluation of oocyte nuclear maturation in young and adult domestic cats (Felis catus). Brazilian Journal of Veterinary Research and Animal Science, 41(4), 268-273. https://doi.org/10.1590/S1413-95962004000400008
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