Leishmania sp. infection in dogs from Florianópolis, Santa Catarina, SC, Brazil

Authors

  • Acácio Duarte Pacheco Universidade Estadual Paulista, Faculdade de Medicina Veterinária, Departamento de Clínica, Cirurgia e Reprodução Animal, Araçatuba, SP
  • Marcia Dalastra Laurenti Universidade de São Paulo, Faculdade de Medicina, Departamento de Patologia, São Paulo, SP
  • Valéria Marçal Félix de Lima Universidade Estadual Paulista, Faculdade de Medicina Veterinária, Departamento de Clínica, Cirurgia e Reprodução Animal, Araçatuba, SP
  • Thaise Yumie Tomokane Universidade de São Paulo, Faculdade de Medicina, Departamento de Patologia, São Paulo, SP
  • Mary Marcondes Universidade Estadual Paulista, Faculdade de Medicina Veterinária, Departamento de Clínica, Cirurgia e Reprodução Animal, Araçatuba, SP

DOI:

https://doi.org/10.11606/issn.1678-4456.v50i3p220-225

Keywords:

Visceral Leishmaniasis, Diagnosis, ELISA, IFAT, PCR

Abstract

The aim of the present study was to investigate the occurrence of Leishmania sp. infection in dogs (N = 491) living in the municipality of Florianópolis, Santa Catarina (SC), Brazil, which was considered a disease-free region for visceral leishmaniasis until 2011, when autochthonous cases of canine disease were notified. Seroprevalence in this population was assessed by ELISA (0.4%; 2/491) and IFAT (4.09%; 24/491). Only one dog exhibited seroreactivity in both serological methods, comprising a total of 25 (5.3%) seroreagent animals. Leishmania sp. DNA, obtained from a sample of whole blood of this animal, was amplified by both conventional and Real-Time PCR. Sequencing of the amplified DNA and, thereby, determination of the Leishmania species involved, was not possible. Our results suggest the necessity of a thorough epidemiological investigation in Florianópolis.

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Published

2013-06-21

How to Cite

Pacheco, A. D., Laurenti, M. D., Lima, V. M. F. de, Tomokane, T. Y., & Marcondes, M. (2013). Leishmania sp. infection in dogs from Florianópolis, Santa Catarina, SC, Brazil. Brazilian Journal of Veterinary Research and Animal Science, 50(3), 220-225. https://doi.org/10.11606/issn.1678-4456.v50i3p220-225

Issue

Vol. 50 No. 3 (2013)

Section

UNDEFINIED

License

The journal content is authorized under the Creative Commons  BY-NC-SA  license (summary of the license: https://creativecommons.org/licenses/by-nc-sa/4.0 | full text of the license: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode) 

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