Lipid structural information from a single equine embryo by MALDI-MS

Authors

  • Roseli Fernandes Gonçalves Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal
  • Christina Ramires Ferreira Aston Laboratory for Mass Spectrometry Laboratory, Chemistry Department, Purdue University, West Lafayette Universidade de Campinas, Instituto de Química, ThoMSon Mass Spectrometry Laboratory
  • Cássia Maria Barroso Orlandi Universidade Estadual Paulista, Faculdade de Medicina Veterinária, Laboratório de Endocrinologia Animal
  • Sérgio Adriano Saraiva Universidade de Campinas, Instituto de Química, ThoMSon Mass Spectrometry Laboratory
  • Heder Nunes Ferreira Universidade Estadual Paulista, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal e Radiologia Veterinária
  • Eduardo Jorge Pilau Universidade de Campinas, Instituto de Química, Dalton Mass Spectrometry Laboratory
  • Fábio César Gozzo Universidade de Campinas, Instituto de Química, Dalton Mass Spectrometry Laboratory
  • Marcos Antônio Achilles Instituto Biológico, Centro de Pesquisa e Desenvolvimento de Sanidade Animal Achilles Genetics
  • Daniela Lazzaro D'Ercole Achilles Genetics
  • Marcos Nogueira Eberlin Universidade de Campinas, Instituto de Química, ThoMSon Mass Spectrometry Laboratory
  • José Antônio Visintin Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal

DOI:

https://doi.org/10.11606/issn.1678-4456.v51i4p340-345

Keywords:

Equine, Embryo, Lipid species, MALDI-MS

Abstract

The aim of this work was to report the potential of MALDI-MS for the characterization of lipid species present in a single equine embryo, and to study some lipid structures detected by collision induced dissociation (CID) experiments. In the positive ion mode spectrum, we could observe mostly protonated and sodiated species of sphingomyelins (SM), phosphatidylcholines (PC) and triacylglycerols (TAG). In the negative ion mode, we observed phosphatidylethanolamines (PE) and phosphatidylinositols (PI). MS/MS spectrum of most intense lipid ions was performed to show MALDI-MS/MS structural information potential. MS/MS spectrum in the positive mode of m/z 760.6 (attributed as PC34:1) depicted characteristic PC fragments of m/z 184.1 (choline polar head), and the neutral loss (NL) of 183 (phosphorylcholine). For the ion of m/z 766.6 (attributed as PE 38:5), we observed the NL of 140, characteristic of PE. For the ion of m/z 808.7 (attributed as PC 38.5), besides the fragment at m/z 184.1 at the NL of 183, it was possible to observe the loss of trimethylamine (ion of m/z 749.6), and the cyclophosphane (ion of m/z 147.0). Finally, for the negative ion mode, we isolated and fragmented the ion at m/z 863.6, which was attributed as PI 36:1 due to the presence of m/z 153 (glycerol phosphate – H2O-H), 223 (phospho inositol – 2H2O-H), 241 (phospho inositol – H2O-H), 281 (oleic acid), and 581.3 (lysophosphoinositol – H2O-H). We conclude that MALDI-MS allowed the detection of a broad range of PC, SM, PE, PI and TAG lipid species, as well as a fast and confident characterization of lipid structures from a single equine embryo. 

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Published

2015-02-06

How to Cite

Gonçalves, R. F., Ferreira, C. R., Orlandi, C. M. B., Saraiva, S. A., Ferreira, H. N., Pilau, E. J., Gozzo, F. C., Achilles, M. A., D’Ercole, D. L., Eberlin, M. N., & Visintin, J. A. (2015). Lipid structural information from a single equine embryo by MALDI-MS. Brazilian Journal of Veterinary Research and Animal Science, 51(4), 340-345. https://doi.org/10.11606/issn.1678-4456.v51i4p340-345

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