Comparing open wound measuring methods popularly used in experimental studies

Authors

  • Ciciane Pereira Marten Fernandes Universidade Federal de Pelotas, Faculdade de Veterinária, Programa de Pós Graduação em Veterinária, Campus Capão do Leão
  • Thiago Vaz Lopes Universidade Federal de Pelotas, Faculdade de Veterinária, Programa de Pós Graduação em Veterinária, Campus Capão do Leão
  • Sabrina de Oliveira Capella Universidade Federal de Pelotas, Faculdade de Veterinária, Programa de Pós Graduação em Veterinária, Campus Capão do Leão
  • Eduardo Garcia Fontoura Universidade Federal de Pelotas, Faculdade de Veterinária, Programa de Pós Graduação em Veterinária, Campus Capão do Leão
  • Mariana Teixeira Tillmann Universidade Federal de Pelotas, Faculdade de Veterinária, Programa de Pós Graduação em Veterinária, Campus Capão do Leão
  • Samuel Rodrigues Félix Universidade Federal de Pelotas, Faculdade de Veterinária, Programa de Pós Graduação em Veterinária, Campus Capão do Leão
  • Márcia de Oliveira Nobre Universidade Federal de Pelotas, Faculdade de Veterinária, Departamento de Clínicas Veterinária, Campus Capão do Leão

DOI:

https://doi.org/10.11606/issn.1678-4456.v52i2p106-111

Keywords:

Healing, Wound contraction, Granulation tissue, Animal experimentation

Abstract

Tissue repair is a response reaction to lesions and aggressions that constitutes a dynamic process to maintain the integrity of the organism. Wound healing experiments have used several approaches in order to assess and compare treatment methods, and these discrepancies hamper comparisons among assays. This study assessed three different methods of wound measurement commonly used in healing assays: clock method, graph paper method, and computer-assisted image analysis. We used 30 Wistar rats, kept in appropriate conditions for animal well-being. After anesthesia, and using an eight-millimeter punch, two lesions were made in the back region of each rat. The wounds were assessed on days four, seven, and 14 after infliction. At four days, all methods generated similar results. By day seven, the clock method had lost precision, likely due to wound shrinkage, and yielded greater means compared to the other two methods. On the last assessment, the computer-assisted method appeared to have more precise results, with the other two generating statistically higher means. Computer-assisted image analysis seems to have maintained wound measuring precision throughout this experiment, even when faced with small lesions. Considering these results, the authors recommend the use of computer-assisted measurements in future experiments.

Downloads

Download data is not yet available.

Author Biography

Ciciane Pereira Marten Fernandes, Universidade Federal de Pelotas, Faculdade de Veterinária, Programa de Pós Graduação em Veterinária, Campus Capão do Leão

Departamento de Clínicas Veterinária

Downloads

Published

2015-06-30

How to Cite

Fernandes, C. P. M., Lopes, T. V., Capella, S. de O., Fontoura, E. G., Tillmann, M. T., Félix, S. R., & Nobre, M. de O. (2015). Comparing open wound measuring methods popularly used in experimental studies. Brazilian Journal of Veterinary Research and Animal Science, 52(2), 106-111. https://doi.org/10.11606/issn.1678-4456.v52i2p106-111

Issue

Section

ARTICLES

Most read articles by the same author(s)