Sensitivity evaluation of the computer-assisted sperm analysis (CASA) in the determination of frozen-thawed bull semen concentration

Authors

  • André Maciel Crespilho VetSemen – Análise de Sêmen para Inseminação Artificial Universidade Santo Amaro, Curso de Medicina Veterinária Universidade Severino Sombra, Curso de Medicina Veterinária
  • Livia Chiaradia Universidade Santo Amaro, Curso de Medicina Veterinária
  • Adriana Cortez Universidade Santo Amaro, Curso de Medicina Veterinária
  • Marcela Vaz Dinelli Universidade Santo Amaro, Curso de Medicina Veterinária
  • Frederico Ozanam Papa Universidade Estadual Paulista, Departamento de Reprodução Animal e Radiologia Veterinária
  • Gustavo Mendes Gomes Universidade Severino Sombra, Curso de Medicina Veterinária
  • Kleber da Cunha Peixoto Júnior Universidade Santo Amaro, Curso de Medicina Veterinária

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2017.127773

Keywords:

Bull semen, CASA, Neubauer, Sperm concentration

Abstract

Sperm concentration is traditionally evaluated by counting cells in a hemocytometric Neubauer chamber, often a highly subjective, time-consuming, and laborious technique prevalent in andrology laboratories around the world. However, the Computer-Assisted Semen Analysis (CASA) represents a more consistent method of evaluating sperm concentration that may provide enhancing efficiencies of sperm count. The purpose of this study is to compare the results of these two methods in the analysis of post-thaw concentration of bovine semen. Four hundred and twenty five batches of semen from different bulls were selected, thawed at 37°C for 30 seconds and then homogenized. Aliquots of 40 μL of semen were diluted in 960 μL of distilled water, fixing the rate at 1:25 dilution for analysis in a Neubauer chamber. Conversely, aliquots of 5 μL for each semen dose were submitted to CASA, considered a minimum of five random fields and 2000 sperm count per analysis. The average concentration of sperm cells was 38.96a ± 1.28 in the Neubauer analysis and 35.14b ± 0.82 for the CASA, with the correlation coefficient of 0.87 (P < 0.0001) and reliability of 0.78 (scale ranging from 0 to 1) between the two methods. In conclusion, the results of two techniques for assessing sperm concentration have similar results. However the CASA methodology would yield greater benefit due to precision, consistency, and reduced disposal issues, particularly for large processing laboratories.

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Author Biography

  • André Maciel Crespilho, VetSemen – Análise de Sêmen para Inseminação Artificial Universidade Santo Amaro, Curso de Medicina Veterinária Universidade Severino Sombra, Curso de Medicina Veterinária
    Departamento de Reprodução Animal, área de Andrologia Veterinária

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Published

2017-11-24

Issue

Section

FULL ARTICLE

How to Cite

Sensitivity evaluation of the computer-assisted sperm analysis (CASA) in the determination of frozen-thawed bull semen concentration. (2017). Brazilian Journal of Veterinary Research and Animal Science, 54(3), 247-252. https://doi.org/10.11606/issn.1678-4456.bjvras.2017.127773