Culture of in vitro produced bovine embryos: effect of number of embryos and medium ratio

Authors

  • Daniela dos Santos Brum Universidade Federal de Santa Maria, Hospital Veterinário, Laboratório de Embriologia Animal, Santa Maria, RS
  • Fábio Gallas Leivas Universidade Federal de Santa Maria, Hospital Veterinário, Laboratório de Embriologia Animal, Santa Maria, RS
  • Mari Lourdes Bernardi Universidade Federal do Rio Grande do Sul, Faculdade de Agronomia, Departamento de Zootecnia, Porto Alegre, RS
  • Fabrício Desconzi Mozzaquatro Universidade Federal de Santa Maria, Hospital Veterinário, Laboratório de Embriologia Animal, Santa Maria, RS
  • Carlos Antonio Mondino Silva Universidade Federal de Santa Maria, Hospital Veterinário, Laboratório de Embriologia Animal, Santa Maria, RS
  • Mara Iolanda Batistella Rubin Universidade Federal de Santa Maria, Hospital Veterinário, Laboratório de Embriologia Animal, Santa Maria, RS

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2006.26493

Keywords:

Bovine embryos, Culture, IVP, OPU

Abstract

In the last decade the dairy and beef bovine farms had an increment in their breeding programs that include the use o ovum pick-up and in vitro production (OPU/IVP). Oocytes retrieved by OPU vary in quality and its reduced number requires appropriated culture conditions for IVP. To evaluate the effect of the number of embryos and volume of the media in the in vitro culture of bovine embryos, 1428 zygotes were cultured in groups of 5, 10 or 20 in 1, 5 or 10mL of medium. Groups of 20 oocytes matured in vitro in 200mL of TCM+rFSHh+ 10% estrus cow serum (ECS). The fertilization was performed in groups of 20 oocytes/200mL of Fert-Talp medium, for 18h with 1x10(6) spermatozoa/mL. The culture was done in SOFaaci+5%ECS for 8 days. Considering D0 as the fertilization day, the IVP was evaluated on day 2 (cleavage), day 7 (blastocyst) and in day 9 (hatching rates). The cleavage rates were higher when embryos were cultured in groups of twenty. However, it was not affected by the medium ratio of 1:1, 1:5 and 1:10. The use of 1:5 and 1:10mL of culture medium ratio showed higher embryo production rates in D7 (P<0.05) than 1:1mL proportion. The culture of 20 embryos per drop affected the blastocyst production on day 7. Likewise the hatching rates in D9 were higher with 1:5 and 1:10 than 1:1. Similarly, the hatching rates were higher in cultured of 10 or 20 embryos/drop when compared to groups of 5 embryos/drop. The reduction of embryos and the proportion of the medium volume in culture affects the development indexes on bovine embryos in vitro production.

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Published

2006-04-01

Issue

Section

UNDEFINIED

How to Cite

Culture of in vitro produced bovine embryos: effect of number of embryos and medium ratio. (2006). Brazilian Journal of Veterinary Research and Animal Science, 43(2), 145-151. https://doi.org/10.11606/issn.1678-4456.bjvras.2006.26493