Leishmania sp. infection in dogs from Florianópolis, Santa Catarina, SC, Brazil

Authors

  • Acácio Duarte Pacheco Universidade Estadual Paulista, Faculdade de Medicina Veterinária, Departamento de Clínica, Cirurgia e Reprodução Animal, Araçatuba, SP
  • Marcia Dalastra Laurenti Universidade de São Paulo, Faculdade de Medicina, Departamento de Patologia, São Paulo, SP
  • Valéria Marçal Félix de Lima Universidade Estadual Paulista, Faculdade de Medicina Veterinária, Departamento de Clínica, Cirurgia e Reprodução Animal, Araçatuba, SP
  • Thaise Yumie Tomokane Universidade de São Paulo, Faculdade de Medicina, Departamento de Patologia, São Paulo, SP
  • Mary Marcondes Universidade Estadual Paulista, Faculdade de Medicina Veterinária, Departamento de Clínica, Cirurgia e Reprodução Animal, Araçatuba, SP

DOI:

https://doi.org/10.11606/issn.1678-4456.v50i3p220-225

Keywords:

Visceral Leishmaniasis, Diagnosis, ELISA, IFAT, PCR

Abstract

The aim of the present study was to investigate the occurrence of Leishmania sp. infection in dogs (N = 491) living in the municipality of Florianópolis, Santa Catarina (SC), Brazil, which was considered a disease-free region for visceral leishmaniasis until 2011, when autochthonous cases of canine disease were notified. Seroprevalence in this population was assessed by ELISA (0.4%; 2/491) and IFAT (4.09%; 24/491). Only one dog exhibited seroreactivity in both serological methods, comprising a total of 25 (5.3%) seroreagent animals. Leishmania sp. DNA, obtained from a sample of whole blood of this animal, was amplified by both conventional and Real-Time PCR. Sequencing of the amplified DNA and, thereby, determination of the Leishmania species involved, was not possible. Our results suggest the necessity of a thorough epidemiological investigation in Florianópolis.

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Published

2013-06-21

Issue

Vol. 50 No. 3 (2013)

Section

UNDEFINIED

License

The journal content is authorized under the Creative Commons  BY-NC-SA  license (summary of the license: https://creativecommons.org/licenses/by-nc-sa/4.0 | full text of the license: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode) 

How to Cite

1.
Pacheco AD, Laurenti MD, Lima VMF de, Tomokane TY, Marcondes M. Leishmania sp. infection in dogs from Florianópolis, Santa Catarina, SC, Brazil. Braz. J. Vet. Res. Anim. Sci. [Internet]. 2013 Jun. 21 [cited 2024 Apr. 19];50(3):220-5. Available from: https://www.revistas.usp.br/bjvras/article/view/64604